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目的和方法:采用MTT 比色法、生长抑制实验、集落形成抑制实验,探讨东亚钳蝎毒抗癌多肽(anticancerpolypeptide from Buthus Martensii Venom ,APBMV) 对Eca 109 细胞和HeLa 细胞的细胞毒作用。结果:APBMV 对Eca109 细胞有明显的毒性作用,且呈显著量效关系,处理细胞24 h 和72 h 的IC50 分别为0-027μg·mL-1 和0-024 μg·mL-1 。APBMV对Eca 109 细胞的生长抑制作用呈明显的时效和量效关系。处理Eca 109 细胞24 h 、48 h 、72 h 的IC50 分别为0-032μg·mL-1,0-028 μg·m L-1 ,0-023 μg·mL-1 。APBMV 还显著抑制Eca109 细胞的集落形成,并有明显的量效关系,其IC50 值为0-023μg·mL-1 。但APBMV 对HeLa 细胞的作用不明显。结论: APBMV 对肿瘤细胞的杀伤或生长抑制作用具有一定的肿瘤差异性。
OBJECTIVE: To investigate the cytotoxic effect of anticancer peptide fromBuhus Martensii Venom (APBMV) on Eca 109 cells and HeLa cells by MTT colorimetric assay, growth inhibition assay and colony formation inhibition assay. Results: APBMV had obvious cytotoxicity on Eca109 cells with significant dose-response relationship. The IC50 of cells treated with APBMV for 24 h and 72 h were 0-027 μg · mL-1 and 0-024 μg · mL-1, respectively. APBMV on the growth inhibition of Eca 109 cells showed significant aging and dose-effect relationship. The IC50 of Eca 109 cells treated with Eca 109 for 24 h, 48 h and 72 h were 0-032 μg · mL -1, 0-028 μg · mL -1, and 0-023 μg · mL -1, respectively. APBMV also significantly inhibited the colony formation of Eca109 cells, and a significant dose-effect relationship, the IC50 value of 0-023μg · mL-1. However, the effect of APBMV on HeLa cells was not obvious. Conclusion: APBMV has some tumor differences on tumor cell killing or growth inhibition.