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目的应用生物信息学分析软件预测结核分枝杆菌MT3876基因编码的蛋白质结构和功能。方法从NCBI数据库获取MT3876基本的基因信息;应用ProParam预测MT3876蛋白的理化性质;应用SignalP 4.0及TMHMM分析其信号肽及跨膜区;利用在线分析Expasy工具分析蛋白二级结构并建立蛋白的三级结构模型;利用ABCpred、SYFPEITHI及NetMHCIIpan 3.1Server分析蛋白抗原表位,寻找最佳B细胞及T细胞抗原位点。结果 MT3876编码结核分枝杆菌假想蛋白MT3876具有172个氨基酸残基。该蛋白无信号肽,位于胞壁,跨膜结构不明显。二级结构中无规卷曲约占52.33%,结构疏松。MT3876具有潜在的B细胞抗原表75~90、103~118、17~32、140~155、96~113、123~142及37~54等位点,T细胞抗原表位1~10、80~88、114~118、133~141及149~172等。结论生物信息学预测MT3876基因编码的蛋白质具有潜在的B、T细胞抗原表位,可作为研发免疫诊断方法的侯选分子靶标。
Objective To predict the structure and function of the protein encoded by Mycobacterium tuberculosis MT3876 using bioinformatics analysis software. Methods To obtain the basic gene information of MT3876 from the NCBI database. ProParam was used to predict the physicochemical properties of MT3876 protein. SignalP 4.0 and TMHMM were used to analyze the signal peptide and transmembrane region. Expasy was used to analyze the protein secondary structure and to establish the protein tertiary Structural model; ABCpred, SYFPEITHI and NetMHCIIpan 3.1Server analysis of protein epitopes, looking for the best B cells and T cell antigenic sites. Results MT3876 encoded M. tuberculosis hypothetical protein MT3876 with 172 amino acid residues. The protein signal peptide, located in the cell wall, transmembrane structure is not obvious. Secondary structure of random curl accounted for about 52.33%, loose structure. MT3876 has the potential B cell epitopes 75-90, 103-118, 17-32, 140-155, 96-113, 123-142, and 37-54. T cell epitopes 1-10, 88,114-118,133-141, 149-172, etc. are mentioned. Conclusion Bioinformatics predicts that the protein encoded by MT3876 gene has potential B and T cell epitopes and can be used as a candidate molecular target for the development of immunodiagnostic methods.