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本文以健康家兔为实验对象,分别采用夹闭肠系膜上动脉所造成的动脉闭塞性(SMAO)休克、自耳静脉注入油酸所复制的急性呼吸窘迫综合征(ARDS)为实验模型,应用“洗肺”技术采取肺巨噬细胞,进行超薄切片和冷冻蚀刻电镜观察;分析比较了肺巨噬细胞在上述实验性疾病时的超微结构变化,探讨了肺巨噬细胞与某些病理过程的关系。一、材料与方法:将健康成年家兔,分为正常对照组,SMAO休克组和ARDS组。各组动物均以适量温生理盐水缓冲液(约50毫升),经气管插管反复灌洗肺组织(六次),再将洗出液三次离心沉淀(500克,8分钟/次),制得5×10~6/毫升肺巨噬细胞混悬液。此后,样品分别经2.5～4.0％戊二醛固定,以618树脂包埋制备超薄切片;经30％甘油生理盐水防冻处理,用日立FHZ—1型冷冻蚀刻装置制得冷冻复型膜。样品送H—500电镜观察,加速电压75KV。
In this paper, healthy rabbits as experimental subjects, respectively, occlusion of the superior mesenteric artery caused by arterial occlusive (SMAO) shock, from the ear vein injection of oleic acid-induced acute respiratory distress syndrome (ARDS) model, the application of “ Lung washing ”technology to take lung macrophages, ultrathin sections and cryo-etching electron microscopy; analysis of lung macrophages ultrastructural changes in the above experimental diseases to explore the relationship between lung macrophages and some pathological processes Relationship. First, materials and methods: The healthy adult rabbits were divided into normal control group, SMAO shock group and ARDS group. The animals in each group were given a moderate amount of warm saline buffer (about 50 ml), lung tissue was repeatedly perfusion through the endotracheal tube (six times), and the eluate was centrifuged three times (500 g for 8 minutes) Have 5 × 10 ~ 6 / ml lung macrophage suspension. Thereafter, the samples were fixed with 2.5-4.0% glutaraldehyde, respectively, and embedded in ultrathin sections with 618 resin. Cryopreservation membranes were obtained by Hitachi FHZ-1 freeze-thawing device with 30% glycerol saline solution. Samples were sent to H-500 electron microscope observation, acceleration voltage 75KV.