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作者在尼泊尔达努萨,鲁潘德希,凯拉利和坎昌普尔4个地区进行了黑热病血清流行病学的调查,这些地区与印度的黑热病流行区比哈尔邦和北方邦毗隣,当地居民主要从事农业劳动,经济生活水平低,人口流动频繁。 191份血清取自肝、脾肿大的非疟疾、结核病、阿米巴病和少数伤寒病病人。用经超声处理的杜氏利什曼前鞭毛体作为 ELISA抗原,测定病人血清中的黑热病抗体,以492nmOD值0.3为阳性。66例病人(34.5％)的
The authors conducted serological surveys of kala-azar in four districts of Nosha, Ruppindeh, Kerala and Kanchipuar in Nepal, bordering Bihar and Uttar Pradesh in India’s endemic area of kala-azar, and local residents mainly engaged in Agricultural labor, low level of economic life and frequent population movements. 191 sera were taken from liver, splenomegaly non-malaria, tuberculosis, amebiasis and a few typhoid patients. The sonicated Leishman’s promastigotes were used as ELISA antigens, and the kala-azar antibody in the patient’s serum was determined to be positive at an OD at 492 nm of 0.3. 66 patients (34.5%)