自1969年丹麦学者 Rygaard 首次将人结肠癌移植于先天免疫缺陷性裸小鼠以来,人类肿瘤异种移植及癌生物学研究有了迅速的发展我们于1990年3月将人结肠腺癌的手术组织移植于裸小鼠皮下及腹腔,现己传至20代,生长稳定,为进一步实验研究奠定了基础。材料和方法一、实验动物:BALB/C nu/nu 裸小鼠,本所裸鼠室提供,鼠龄4—8周,雌雄兼用,实验鼠饲养于超净室中,经灭菌处理的水和饲料供 Since the Danish scholar Rygaard first transplanted human colon cancer into innate immunodeficient nude mice in 1969, human tumor xenotransplantation and cancer biology research has developed rapidly. We have performed surgical treatment of human colon adenocarcinoma in March 1990. Transplanted in the subcutaneous and abdominal cavity of nude mice, it has been transmitted to the 20th generation and has stable growth. It laid the foundation for further experimental research. Materials and Methods 1. Experimental animals: BALB/C nu/nu nude mice, provided by our nude mouse room, 4 to 8 weeks of age, both male and female, and experimental rats raised in a clean room, sterile water And feed supply