论文部分内容阅读
目的 研究流式细胞仪 (FCM)检测肺癌穿刺标本p5 3蛋白、增殖细胞核抗原 (PCNA)表达的价值。资料与方法 运用FCM检测 6 6例穿刺标本p5 3蛋白、PCNA表达。结果 (1) 6 6例肺肿块中病理诊断恶性 5 3例 ,13例病理诊断及临床随防诊断为良性病变。 (2 ) 5 3例肺恶性病变p5 3蛋白表达阳性率为 6 2 .3% ,PCNA阳性率为4 5 .3% ;13例良性病变p5 3蛋白表达阳性率为 30 .8% ,PCNA阳性率为 7.7% ;两组p5 3蛋白、PCNA阳性表达有显著性差异 (P <0 .0 5 )。 (3) 5 3例肺恶性病变穿刺敏感性为 94 .3% ,13例肺良性病变穿刺特异性为 76 .9% ,6 6例穿刺诊断准确性为 90 .9% ,气胸发生率为 4 .6 %。结论 运用FCM可以检测肺癌穿刺标本p5 3蛋白、PCNA表达 ,为了解肺癌的分子生物学行为特性提供帮助。
Objective To investigate the expression of p53 protein and proliferating cell nuclear antigen (PCNA) in lung cancer specimens by flow cytometry (FCM). Materials and Methods 6 cases of puncture specimens p5 3 protein and PCNA were detected by FCM. Results (1) Pathological diagnosis of 56 cases of lung mass in 53 cases, 13 cases of pathological diagnosis and clinical diagnosis as benign lesions. (2) The positive rate of p53 protein in 53 cases of lung malignant lesions was 62.3%, the positive rate of PCNA was 45.3%. The positive rate of p5-3 protein in 13 cases of benign lesions was 30.8%, and the positive rate of PCNA was The rate was 7.7%. There was a significant difference between the two groups in the expression of p53 protein and PCNA (P <0.05). (3) The sensitivity of puncturing in 53 cases of lung malignant lesions was 94.3%, the specificity of puncturing in 13 cases of benign lung diseases was 76.9%, the diagnostic accuracy of 66 cases in puncture was 90.9%, and the incidence of pneumothorax was 4 .6%. Conclusion FCM can detect the expression of p53 protein and PCNA in lung cancer specimens and help to understand the molecular biological behavior of lung cancer.