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用ＰＣＲ－ＳＳＣＰ方法对３０例大肠癌组织ｐ５３基因突变状况进行了检测。结果表明：３０例大肠癌ｐ５３基因的突变率为２０％（６／３０），其中１例在第４外显子，５例在第５外显子，第２、３外显子未检出突变。高分化和中等分化肠癌ｐ５３基因突变率均为１／８（１２．５％），低分化为３／１０（３０．０％），未分化为１／４（２５．０％）；在Ｄｕｋｅｓ分期中的突变率分别为：Ｂ２期为１／１０（１０．０％），Ｃ１期为２／１２（１６．７％），Ｄ期为３／８（３７．５％）。结果提示ｐ５３基因突变与Ｄｕｃｋｅｓ分期有关，且肠癌的ｐ５３基因突变很少发生在第２、３外显子
PCR-SSCP method was used to detect the mutation of p53 gene in 30 cases of colorectal cancer. The results showed that the mutation rate of p53 gene in 30 cases of colorectal cancer was 20% (6/30), of which 1 was in exon 4, 5 was in exon 5, exon 2 and 3 were not detected. mutation. The mutation rate of p53 in well-differentiated and moderately differentiated colon cancers was 1/8 (12.5%), poorly differentiated to 3/10 (30.0%), and undifferentiated to 1/4 (25.0%); The mutation rates in the Dukes stage were 1/10 (10.0%) in the B2 stage, 2/12 (16.7%) in the C1 stage, and 3/8 (37.5%) in the D stage. The results suggest that the p53 gene mutation is related to the Duckes staging stage, and the p53 gene mutation in the colon cancer rarely occurs in exon 2 and exon 3