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目的 研究c—erbB—2和c—myc癌基因在人胃癌组织中的扩增意义。方法 应用原位杂交技术检测35例胃癌标本中c—erbB—2和c—myc的扩增情况,同时应用斑点杂交技术和免疫组化技术(ABC法)检测人胃癌NKM—45细胞中c—erbB—2、P53、P21和P62~(c-myc)的扩增和过度表达情况。结果 c—erbB—2和c—myc在人胃癌中的扩增率分别为31.4％和37.1％,两基因的扩增具有显著相关性(P<0.005),并发现在NKM—45细胞中同时有P53、P21、P62~(c-myc)过度表达和c—erbB—2扩增。结论 c—erbB—2和c—myc基因扩增及P53、P21、P62~(c-myc)过度表达是胃癌发生过程中的一个重要的生物学因素。
Objective To investigate the significance of amplification of c-erbB-2 and c-myc oncogenes in human gastric cancer tissues. Methods In situ hybridization was used to detect the amplification of c-erbB-2 and c-myc in 35 specimens of gastric cancer. The dot blot technique and immunohistochemical technique (ABC method) were used to detect c-myc in human gastric cancer. The amplification and overexpression of erbB-2, P53, P21 and P62-(c-myc). Results The amplification rates of c-erbB-2 and c-myc in human gastric cancer were 31.4% and 37.1%, respectively. There was a significant correlation between the amplification of the two genes (P<0.005) and they were found in NKM-45 cells. P53, P21, P62~(c-myc) overexpression and c-erbB-2 amplification. Conclusion The amplification of c-erbB-2 and c-myc genes and overexpression of P53, P21 and P62 c-myc are important biological factors in the development of gastric cancer.