本文应用免疫组织化学ＡＢＣ方法，对３６例胆囊癌组织标本进行了癌基因ｃｅｒｂＢ２的测定，同时进行了增殖细胞核抗原（ＰＣＮＡ）的测定，作为反映ｃｅｒｂＢ２的表达与胆囊癌细胞增殖状态关系的指标，结果３６例胆囊癌组织中，１４例（３８．８８％）显示ｃｅｒｂＢ２基因过度表达；２２例（６１．１１％）显示ＰＣＮＡ高表达，其中１２例两种标记同时阳性。半定量分级，Ｓｐｅａｒｍａｎ秩相关分析表明，两者呈显著相关（Ｐ＜０．０１）；ｃｅｒｂＢ２基因表达的程度与胆囊癌的组织学类型有关。本文结果提示，胆囊癌中有ｃｅｒｂＢ２基因的异常表达并可能有病因学意义；ｃｅｒｂＢ２基因的表达情况可有效地反映胆囊癌的生物学特性，作为衡量胆囊癌分化程度、判断患者预后的有用指标 In this study, the c-erbB2 oncogene was detected in 36 specimens of gallbladder carcinoma by immunohistochemical ABC method. At the same time, proliferating cell nuclear antigen (PCNA) was measured to reflect the expression of cerbB2 and gallbladder. Indices of the relationship between cancer cell proliferation status, results 36 cases of gallbladder carcinoma, 14 cases (38.88%) showed c erbB  2 gene overexpression; 22 cases (61.11%) showed high expression of PCNA, including 12 cases Both markers are positive at the same time. Semi-quantitative grading, Spearman rank correlation analysis showed that the two were significantly correlated (P <0.01); the degree of c erbB 2 gene expression was related to the histological type of gallbladder cancer. The results of this study suggest that there is abnormal expression of cerbB2 in gallbladder carcinoma and may have etiological significance. The expression of cerbB2 gene can effectively reflect the biological characteristics of gallbladder carcinoma as a measure of gallbladder cancer differentiation. Useful indicators for judging the prognosis of patients