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转移因子,虽然在临床上已应用于免疫缺陷病、感染性疾病、癌症和其他疾患的治疗,但关于TF、对免疫应答的影响问题尚未见较多的报道,为了阐明人脐血在TF和丝裂原作用下的淋巴细胞转化,我们利用~3H—TdR掺入法比较32份脐血标本对TF—h和TF—P的反应,们们试验证明,32份标本中有4份,当分别加入0、1m 1的TF—h或TF—P,培养72小时后,表现了淋转的增加,尽管TF的这种丝裂原样活性相对低些。我们还发现,当加入TF时,13份PHA诱导的淋巴细胞转化受到抑制,但8份标本加TF培养后促进了PHA诱导的淋巴细胞转化。此外,我们观察到有8份脐血既不受TF—h加PHA,也不受TF—P加PHA的影响。我们观察到,在TF—h加PHA组,和PHA组之间,淋巴细胞转化无明显差异(P>0.05)。但是,在TF—P加PHA组,和PHA组诱导淋巴细胞转化作用之间却有统计学意义。前者,即猪的转移因子,抑制了PHA的刺激作用(P<0.01)。这些资料提示,TF—h和TF—P制剂,既含有刺激的成分,又含有抑制淋转的成分。同时说明,人脐血淋巴细胞对TF—h及TF—P具有复杂的个体反应性。
Although TFs have been used clinically in the treatment of immunodeficiency diseases, infectious diseases, cancers and other diseases, there is not much report on the impact of TF on immune response. To clarify the role of TF in human umbilical cord blood We used ~ 3H-TdR incorporation to compare the response of 32 cord blood samples to TF-h and TF-P. We have shown that 4 out of 32 samples Addition of 0,1 m <1> of TF-h or TF-P, respectively, after 72 hours of culture showed an increase in lymphatic transit, although this mitochondrial-like activity of TF was relatively low. We also found that 13 PHA-induced lymphocyte transformation was inhibited when TF was added, but 8 of the samples plus TF cultured promoted PHA-induced lymphocyte transformation. In addition, we observed that 8 cord blood samples were neither affected by TF-h plus PHA nor by TF-P plus PHA. We observed no significant difference in lymphocyte transformation between TF-h plus PHA group and PHA group (P> 0.05). However, there was a significant difference between TF-P plus PHA group and PHA group in inducing lymphocyte transformation. The former, the pig transfer factor, suppresses the stimulation of PHA (P <0.01). These data suggest that TF-h and TF-P preparations contain both stimulating and anti-leaching ingredients. At the same time, human cord blood lymphocytes have complex individual reactivity to TF-h and TF-P.