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目的:人类精子对氧化应激非常敏感,解偶联蛋白2(UCP2)存在于多种组织内,通过控制细胞内活性氧(ROS)水平,参与细胞抗氧化应激损伤。本研究通过观察精子UCP2表达对精子膜脂类过氧化物水平和活力的影响,探讨精子的抗氧化机制。方法:应用计算机辅助精液分析对97份精液参数检测,根据a+b级精子百分率分为4组,Ⅰ组25例:a+b级精子>50%;Ⅱ组24例:a+b级精子25%～50%;Ⅲ组24例:a+b级精子10%～25%;Ⅳ组24例:a+b级精子<10%。应用RT-PCR检测精子UCP2mRNA的表达;同时测定精子悬液中膜脂类过氧化反应产物丙二醛(MDA)含量。结果:4组精子UCP2mRNA表达水平分别为1.51±0.24、1.28±0.15、1.17±0.20、0.69±0.18,MDA含量分别为(14.66±2.55)、(16.00±2.09)、(17.44±1.40)、(21.20±3.50)nmol/108精子。UCP2mRNA表达与MDA含量呈负相关(r=-0.633,P<0.01),精子活力较低组UCP2表达显著下降(P<0.01),MDA含量显著升高(P<0.05)。结论:精子内存在UCP2-ROS调节系统,UCP2参与精子氧化还原调节,在精子抗氧化应激中起重要作用。
OBJECTIVE: Human spermatozoa are very sensitive to oxidative stress. Uncoupling protein 2 (UCP2) exists in many tissues and is involved in the anti-oxidative stress injury of cells by controlling the level of reactive oxygen species (ROS) in cells. In this study, the effects of sperm UCP2 expression on lipid peroxidation and sperm motility of sperm were observed to explore the anti-oxidative mechanism of sperm. Methods: 97 sperm parameters were detected by computer-aided semen analysis, divided into 4 groups according to a + b grade sperm percentage, 25 cases in group Ⅰ: a + b grade sperm> 50%; group Ⅱ 24 cases: a + b grade sperm 25% ~ 50%; group Ⅲ 24 cases: a + b sperm 10% ~ 25%; Ⅳ group 24 cases: a + b sperm <10%. The expression of UCP2 mRNA in spermatozoa was detected by RT-PCR. The content of malondialdehyde (MDA), a membrane lipid peroxidation product, in sperm suspension was also determined. Results: The expression levels of UCP2 mRNA in the 4 groups were 1.51 ± 0.24, 1.28 ± 0.15, 1.17 ± 0.20 and 0.69 ± 0.18 respectively, and the contents of MDA in the 4 groups were (14.66 ± 2.55), (16.00 ± 2.09), (17.44 ± 1.40) and (21.20 ± 3.50) nmol / 108 sperm. UCP2 mRNA expression was negatively correlated with MDA content (r = -0.633, P <0.01). UCP2 expression was significantly decreased (P <0.01) and MDA content was significantly increased (P <0.05). CONCLUSIONS: UCP2-ROS regulatory system exists in sperm, and UCP2 is involved in the regulation of redox regulation of sperm, which plays an important role in anti-oxidative stress in sperm.