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目的:探讨口腔鳞癌细胞中β-DG蛋白的存在状态及其分子机制。方法:在检测口腔鳞癌四株细胞系中β-DG蛋白存在状态的基础上,应用Western blot和蛋白质测序法研究基质金属蛋白酶(MMP)抑制剂1,10-phenanthroline对β-DG蛋白降解和癌细胞侵袭能力的逆转作用。结果:口腔鳞癌细胞中β-DG蛋白发生了降解,经MMP抑制剂1,10-phenanthroline作用后可以逆转β-DG蛋白降解和降低癌细胞的侵袭能力。蛋白测序证实,β-DG蛋白降解片段N端的氨基酸顺序为Ile-Asn-Thr-Asn或Ile-Val-Thr-Gln。结论:口腔鳞癌细胞中β-DG蛋白降解与癌细胞的侵袭和转移能力有关,MMP的剪切作用可能是β-DG蛋白降解的重要机制之一。
Objective: To investigate the existence and molecular mechanism of β-DG in oral squamous cell carcinoma. METHODS: Based on the detection of the presence of β-DG in four cell lines of oral squamous cell carcinoma, the effects of 1,10-phenanthroline, a matrix metalloproteinase (MMP) inhibitor, on the degradation of β-DG protein and protein expression were investigated by Western blot and protein sequencing Cancer cell invasion ability of the reversal effect. Results: The β-DG protein in oral squamous cell carcinoma cells was degraded. After treated with 1,10-phenanthroline, MMP-1 could reverse the degradation of β-DG protein and reduce the invasiveness of cancer cells. Protein sequencing confirmed that the amino acid sequence at the N-terminus of the β-DG proteolytic fragment was Ile-Asn-Thr-Asn or Ile-Val-Thr-Gln. Conclusion: The degradation of β-DG in oral squamous cell carcinoma is related to the invasion and metastasis of cancer cells. The shearing effect of MMP might be one of the important mechanisms of β-DG degradation.