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应用离体叶片点滴接种平脐蠕孢菌Bipolaris sorokiniana孢子悬浮液并进行电镜观察,研究小麦及其近缘种属叶片上病斑型的发生特点及病、健组织细胞内部超微结构的变化。结果表明,不同品种小麦相同发育阶段的离体叶上病斑型的变化仅与其自身抗病性遗传表达有关。小麦病斑的反应型变化过程,随时间在品种、叶位、不同发育阶段间存在差异,叶肉病变细胞中细胞器结构及功能完好的为14.3%,变化的为28.6%,解体和功能丧失的为57.1%;小麦近缘种病斑的反应型不随时间改变,仅在种属间存在差异,且叶位间的差异不显著,大赖草病变细胞中细胞器结构及功能完好、改变和丧失的均为33.3%。
In vitro leaves were inoculated with Bipolaris sorokiniana spore suspension and observed with electron microscopy to study the occurrence characteristics of diseased plaques on leaves of wheat and its related species and the ultrastructural changes of diseased and healthy tissue cells. The results showed that the variation of diseased leaf spot patterns in isolated leaves of different wheat cultivars at the same development stage was only related to the genetic expression of their own disease resistance. The reaction type of wheat lesion varied with time at the variety, leaf position and different developmental stages. The structure and function of organelles in the diseased mesophyll cells were 14.3% and 28.6%, respectively. The disintegration and loss of function were 57.1%. The reaction type of lesion in wheat closely related species did not change with time, and there was only difference among species, and the difference between leaf positions was not significant. The structure and function of organelles in Lespedeza herbae cells were intact, 33.3%.