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目的:探讨研究克拉霉素对小鼠动脉粥样硬化炎症反应的影响。方法:选择雄性的C57BL/6J小鼠40只为研究对象,通过建模分为对照组(10只)和实验组(30只),利用肺炎衣原体反复感染,同时给予高胆固醇食物饲养的方法,成功建立了动脉粥样硬化炎症模型。依据有无克拉霉素治疗及治疗的时间进一步分为未治疗亚组、早期治疗亚组、延迟治疗亚组各10只。数理统计分析各组的IL-6、IL-8水平及主动脉粥样硬化的治疗情况。结果:实验组小鼠的LDL-C、TG、TC水平显著高于对照组的(P<0.05),且均显示Cpn IgG抗体的阳性结果。与对照组相比,实验组小鼠的IL-6、IL-8水平显著升高(P<0.05)。实验组内IL-6、IL-8水平比较:早期治疗亚组、延迟治疗亚组的显著低于未治疗亚组(P<0.05),且早期治疗亚组的显著低于延迟治疗亚组(P<0.05)。未治疗亚组的动脉损伤率为100%,明显高于早期治疗亚组的20.00%及延迟治疗亚组的33.33%(P<0.05)。结论:早期克拉霉素治疗可以有效地减轻小鼠动脉粥样硬化炎症反应,延缓动脉粥样硬化的发生。
Objective: To investigate the impact of clarithromycin on atherosclerosis inflammatory reaction in mice. Methods: Forty male C57BL / 6J mice were selected as the control group, and were divided into control group (n = 10) and experimental group (n = 30) by modeling. Chlamydia pneumoniae was repeatedly infected with high- Successful establishment of an atherosclerotic inflammation model. Depending on whether or not Clarithromycin treatment and treatment time is further divided into untreated subgroup, early treatment subgroup, delayed treatment subgroup of 10. The levels of IL-6 and IL-8 in each group and the treatment of aortic atherosclerosis were analyzed by mathematical statistics. Results: The levels of LDL-C, TG and TC in the experimental group were significantly higher than those in the control group (P <0.05), and all showed positive results of Cpn IgG antibody. Compared with the control group, the experimental group of mice IL-6, IL-8 levels were significantly increased (P <0.05). The levels of IL-6 and IL-8 in the experimental group were significantly lower than those in the untreated group (P <0.05) in the early treatment subgroup and in the delayed treatment subgroup, and were significantly lower in the early treatment subgroup than in the delayed treatment subgroup P <0.05). The rate of arterial injury in the untreated subgroup was 100%, significantly higher than 20.00% in the early subgroup and 33.33% in the delayed subgroup (P <0.05). Conclusion: Early clarithromycin treatment can effectively reduce the atherosclerosis inflammation in mice and delay the occurrence of atherosclerosis.