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利用化学共沉淀法制备近微米级葡聚糖T-40包被的超顺磁纳米氧化铁(SPIO)粒子。用X射线衍射(XRD)、透射电镜(TEM)、原子力显微镜(AFM)、红外光谱等手段对其性质进行表征。XRD确定所制备的粒子主要为Fe3O4晶体,具有超顺磁性且饱和磁化强度为64.396emu/g;TEM显示无机核心粒径约为20nm;被葡聚糖包被后,AFM显示粒子呈扁平长方体形貌,三维尺寸为(200～300)nm×(400～600)nm×(50～70)nm。在超声作用下,该类粒子能够对小鼠H-22肝癌细胞进行标记,说明所制备的磁性纳米粒子具有作细胞磁性标记物的特性,同时超声也可促进H-22细胞快速装载磁性微粒。
Nearly micron-sized dextran T-40 coated superparamagnetic iron oxide (SPIO) particles were prepared by chemical coprecipitation. Its properties were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), atomic force microscopy (AFM) and infrared spectroscopy. XRD confirmed that the prepared particles were mainly Fe3O4 crystals with superparamagnetism and saturation magnetization of 64.396emu / g; TEM showed that the diameter of inorganic core was about 20nm; AFM showed the particles were flat rectangular shape after being coated with dextran The appearance and the three-dimensional size are (200-300) nm (400-600) nm (50-70) nm. Under the action of ultrasound, this kind of particles could mark the H-22 hepatocarcinoma cells in mice, which showed that the prepared magnetic nanoparticles had the characteristics of magnetic markers for cells. Meanwhile, ultrasound could also promote the rapid loading of H-22 cells with magnetic particles.