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目的:研究七叶莲花的抗炎镇痛作用及其可能作用机制。方法:通过醋酸致小鼠扭体试验、热板试验、福尔马林试验、二甲苯致小鼠耳肿胀试验和大鼠胸膜炎模型,观察七叶莲花的抗炎镇痛作用;采用紫外分光光度法测定血清前列腺素E2(PGE2)、丙二醛(MDA)和一氧化氮(NO)及细胞因子(IL-1β、IL-6和TNF-α)的含量。结果:七叶莲花可减少醋酸致小鼠扭体次数、增加热板小鼠痛阈值、抑制福尔马林实验、二甲苯致小鼠耳肿胀及大鼠胸膜炎炎症反应,降低动物血清PGE2、MDA、NO及相关细胞因子水平。结论:七叶莲花乙醇粗提物有显著抗炎镇痛作用,作用机制可能与降低相关炎症介质及细胞因子水平有关。
Objective: To study the anti-inflammatory and analgesic effect of Aesculapius and its possible mechanism. Methods: Anti-inflammatory and analgesic effects of seven-leaf lotus were observed by acetic acid-induced writhing test, hot plate test, formalin test, xylene-induced mouse ear swelling test and rat pleurisy model. UV spectrophotometry (PGE2), malondialdehyde (MDA), nitric oxide (NO), and cytokines (IL-1β, IL-6 and TNF-α) Results: Aescin can reduce the number of writhing induced by acetic acid in mice, increase the pain threshold of hot plate mice, inhibit formalin test, xylene-induced ear swelling in mice and inflammatory reaction of pleuritis in rats, reduce the levels of PGE2, MDA , NO and related cytokine levels. Conclusion: Ethanol extract of Aescula lotus has significant anti-inflammatory and analgesic effects, and its mechanism may be related to the reduction of the relevant inflammatory mediators and cytokines.