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背景:近年来研究表明神经节苷脂(gangliosides,GM-1)能够修复中枢神经系统中神经元的损伤。目的:观察不同剂量神经节苷脂(GM-1)对神经干细胞增殖和分化的影响。设计:完全随机对照的开放实验。地点与材料:研究地点为郑州市第二人民医院脑外科和郑州大学医学生物工程研究所。材料为胎龄10周流产胚胎脑组织。方法与主要观察指标:从人胚胎脑组织分离出神经干细胞,培养于含bFGF和B27的DMEM/F12细胞营养液中,实验组加入不同浓度的GM-1,用MTT比色分析法测定细胞增殖能力。用含3%血清的DMEM/F12营养液诱导细胞分化,每间隔6h于倒置相差显微镜下观察细胞分化情况。结果:5d,10dMTT比色显示:bFGF(20mg/L)+B27+DMEM/F12和GM-1(33.5mg/L)+B27+DMEM/F12两组间t检验,P值均>0.05;GM-1(33.5mg/L)+B27+DMEM/F12与B27+DMEM/F12两组间t检验,P值均<0.05;GM-1(0.335mg/L)+B27+DMEM/F12,GM-1(3.35mg/L)+B27+DMEM/F12与B27+DMEM/F12间t检验,P值均>0.05。分化实验发现,接种6h后,(3%)血清+DMEM/F12组神经球周边可见明显的细胞突起,呈放射状向周围伸出,而3个实验组和阴性对照组的细胞突起短且细小,随着培养时间的延长,各组的细胞突起均逐渐伸长,3个实验组分化能力低于对照组,与阴性对照组之间无明显差异。结论:GM-1能够维持神经干细?
BACKGROUND: In recent years, studies have shown that gangliosides (GM-1) can repair neuronal damage in the central nervous system. Objective: To observe the effects of different doses of gangliosides (GM-1) on the proliferation and differentiation of neural stem cells. Design: A fully randomized controlled open experiment. Location and Materials: The study site was located in Zhengzhou Second People’s Hospital Department of Brain Surgery and Zhengzhou University Institute of Medical Bioengineering. Materials for gestational age 10 aborted embryonic brain tissue. METHODS AND MAIN OUTCOME MEASURES: Neural stem cells were isolated from human embryonic brain tissue, cultured in nutrient solution containing DMEM / F12 cells containing bFGF and B27, and different concentrations of GM-1 were added to the experimental group. Cell proliferation was measured by MTT colorimetric assay ability. Cell differentiation was induced by DMEM / F12 nutrient solution containing 3% serum, and cell differentiation was observed under an inverted phase contrast microscope at intervals of 6 hours. Results: The colorimetric results at 5d and 10d showed that t-test between bFGF (20mg / L) + B27 + DMEM / F12 and GM-1 (33.5mg / L) (P <0.05); The levels of GM-1 (0.335 mg / L) + B27 + DMEM / F12 and GM- 1 (3.35mg / L) + B27 + DMEM / F12 and B27 + DMEM / F12 t tests, P values were> 0.05. Differentiation experiments showed that 6h after inoculation, (3%) serum + DMEM / F12 group visible around the neurospheres prominent cell protrusions radially extended around the three experimental group and the negative control group of cells protruding short and small, With the prolongation of culture time, the cell protrusion of each group gradually extended. The differentiation ability of the three experimental groups was lower than that of the control group, and there was no significant difference with the negative control group. Conclusion: GM-1 can maintain the nervous fine?