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目的：建立新生大鼠缺氧缺血性脑损伤（HIBD）模型，研究HIBD时脑组织中HIF-1α和VEGF的表达及其相关性，为临床治疗提供实验依据。方法7日龄新生Wistar大鼠112只，随机分为正常组，HIBD模型组。各组分别于规定时间点取脑组织，免疫组织化学方法检测HIF-1α和VEGF的阳性表达及其相关性。结果（1）HIBD模型组HIF-lα及VEGF阳性表达与正常组比较，差异均有统计学意义（P<0.05）。（2）HIBD模型组HIF-1α和VEGF的表达趋势一致，呈直线正相关（r=0.613，P＜0.01）。结论 HIF-1α和VEGF在HIBD新生大鼠神经保护作用中起协同作用。“,”Objective To investigate the expression of HIF-1ain and VEGF in Hypoxia Ischemia Brain Damage in neonatal rats.Methods A total of 112 female or male Wistar rats aged 7 days were randomly divided into three groups:normol group and HIBD model group.HIBD model of neonatal rats were established in HIBD model group. Rats in three groups were sacrificed at different time points and brain tissue were collected.Then morphologic changes of brain tissue in general obtained through macroscopic observation, the brain tissue were HE stained to observe the pathological changes under light microscope.The expression of HIF-1α and VEGF in hippocampa was detected with the immunohistochemistry method.Results (1)In the Hypoxia Ischemia Brain Damage model, the expression of HIF-1α and VEGF positive cells compared with the normol group (P<0.05).(2)The expressions of HIF-1α and VEGF in brain tissue in HIBD had notable positive correlation (r=0.613,P<0.01).Conclusion The expressions of HIF-1α and VEGF in brain tissue in HIBD and rhG-CSF treatment group had notable positive correlation.