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目的应用扫描电镜观察亚洲带绦虫囊尾蚴的超微结构。方法将亚洲带绦虫囊尾蚴进行清洗,戊二醛和四氧化锇固定,乙醇梯度脱水,乙酸异戊酯置换,CO2临界点干燥后喷金镀膜,用扫描电镜观察超微结构并记录。结果亚洲带绦虫囊尾蚴分为头节、颈部和囊体三部分。头节呈四棱台形,侧面观察呈方形,有1个顶突和4个吸盘。吸盘呈洞穴状,表面有皱褶,且布满粗短钝圆的微毛。颈部较短,直径约为头节的一半,中部较窄,因皮肌的收缩出现大量环形邹褶和纵型皱褶,颈部微毛较稀疏,有棘样尖端。囊体呈椭圆形,表面粗糙不平,呈丘陵状起伏。囊部微毛密集、细长,呈刷状或网状,末端可见圆形分泌颗粒。结论亚洲带绦虫囊尾蚴具顶突、吸盘、微毛等特有超微结构,这些超微结构与附着、运动和营养吸收等生理功能有密切联系,在生物学中具有重要意义。
Objective To observe the ultrastructure of Cysticercus cellulosae in Asia using scanning electron microscopy. Methods Cysticercus cellulosae was cleaned, fixed with glutaraldehyde and osmium tetroxide, dehydrated in ethanol and replaced with isoamyl acetate. The critical point of CO2 was sprayed on the membrane and the ultrastructure was observed with scanning electron microscopy. Results Cysticercus cellulosae in Asia were divided into three sections: head, neck and capsule. The head section has a quadrangular trapezoid, the side of the square was observed, there is a top and 4 sucker. Sucker was cave-shaped, the surface of folds, and covered with stubby blunt hair. The neck is shorter, about half the diameter of the head, the middle is narrower, a large number of circular Zou folds and vertical folds due to the contraction of the skin muscle, neck sparsely hairy, spiky tip. Capsule was oval, rough surface uneven, was rolling hills. Cap hair micro-dense, slender, was brush-like or mesh, the end of the circular particles can be seen secretory. CONCLUSIONS Cysticercus cellulosae in Asia has unique ultrastructures such as maxillary processes, sucker and micro-hair. These ultrastructures are closely related to physiological functions such as attachment, exercise and nutrient absorption, and are of great significance in biology.