代谢组学是上世纪90年代中期发展起来的研究细胞和生物体的所有代谢中间体和终产物的一门新兴学科。其研究对象一般为分子量在1 000Da以下的小分子。代谢组学通过对生物体系中的小分子化合物进行定性定量分析,解析生物体系的代谢途径并揭示内源性小分子化合物与毒性、疾病、生命活动规律等的相互关系。本文综述代谢组学的概况及其在法医毒理学中的应用现状,为相关研究和应用提供参考。 Metabolomics is a new discipline developed in the mid-1990s to study all metabolic intermediates and end products of cells and organisms. Its research object is generally a small molecule with a molecular weight below 1 000 Da. Metabolomics, through the qualitative and quantitative analysis of small molecule compounds in biological systems, analyzes the metabolic pathway of biological systems and reveals the correlation between endogenous small molecule compounds and toxicity, disease and life activity rules. This review summarizes the general situation of metabolomics and its application in forensic toxicology and provides references for related research and application.