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目的:观察醒脑静注射液对严重脓毒症大鼠血清细胞因子即肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的影响。方法:按照随机数字表法将90只SD大鼠随机分为9组,每组各10只:脂多糖(LPS)6 h组、LPS 24 h组、LPS 48 h组、XNJ 6 h组、XNJ 24 h组、XNJ 48 h组、对照6 h组、对照24 h组、对照48 h组。各LPS组、各XNJ组以腹腔注射LPS建立严重脓毒症模型,各XNJ组尾静脉注入醒脑静注射液。用酶联免疫吸附(ELISA)法检测9组大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)水平。结果:各LPS组和各XNJ组不同时间点的CRP浓度随时间延长呈上升趋势,在48 h达到高峰,与各对照组大鼠比较,各LPS组和各XNJ组血清CRP水平升高显著(P<0.05)。各XNJ组不同时间血清CRP浓度较各LPS组为低(P<0.05)。与对照组大鼠比较,各LPS组和各XNJ组血清TNF-α、IL-6和IL-1β水平升高显著(P<0.05),6 h达高峰,6 h后开始下降。各XNJ组各时间点TNF-α、IL-6和IL-1β水平与各LPS组比较,有显著降低(P<0.05)。结论:醒脑静注射液可显著降低严重脓毒症大鼠部分促炎因子的水平并在早期即开始起抑制作用,从而可能起到减轻严重脓毒症炎症反应的作用。
Objective: To observe the effects of xingnaojing injection on serum levels of cytokine TNF-α, IL-1β, IL-6 )Impact. Methods: Ninety Sprague-Dawley rats were randomly divided into 9 groups (n = 10): LPS for 6 h, LPS for 24 h, LPS for 48 h, XNJ for 6 h, XNJ 24 h group, 48 h XNJ group, 6 h control group, 24 h control group and 48 h control group. Each LPS group and each XNJ group were given intraperitoneal injection of LPS to establish a model of severe sepsis, and each XNJ group was injected with Xingnaojing injection through tail vein. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were detected by enzyme linked immunosorbent assay (ELISA) Results: The CRP levels in different LPS groups and different XNJ groups at different time points increased with the increase of time and peaked at 48 h. Serum CRP levels in each LPS group and each XNJ group were significantly higher than those in the control groups P <0.05). The serum CRP levels in different XNJ groups at different time points were lower than those in LPS group (P <0.05). Compared with the control group, the levels of TNF-α, IL-6 and IL-1β in each LPS group and each XNJ group increased significantly (P <0.05), peaked at 6 h, and then decreased 6 h later. The levels of TNF-α, IL-6 and IL-1β at each time point in each XNJ group were significantly lower than those in LPS group (P <0.05). Conclusion: Xingnaojing injection can significantly reduce the levels of some proinflammatory cytokines in rats with severe sepsis and begin to inhibit early, which may play a role in alleviating the severe sepsis inflammatory reaction.