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目的:研究线粒体数目与肝癌生长的相关性。方法:利用电子显微镜技术定量肝癌组织中线粒体数目,利用免疫组织化学技术评估肝癌组织中的线粒体标志物COX Ⅳ及HSP60表达水平,分析电镜肝癌线粒体定量结果与COX Ⅳ及HSP60表达量之间的相关性,并比较肝癌中线粒体数目、COX Ⅳ及HSP60表达量与肝癌直径之间的关系。结果:与癌旁相比,肝癌组织中线粒体数目(P<0.001)、COX Ⅳ及HSP60的表达量显著降低(P=0.0417,P=0.0290)。COX Ⅳ及HSP60表达水平与线粒体数目无显著相关(r~2=0.009,P=0.5468;r~2=0.056,P=0.1396)。线粒体数目与肿瘤直径显著负相关(r~2=0.1086,P=0.0434),COX Ⅳ及HSP60表达量与肿瘤直径无显著相关(r~2=0.0251,P=0.3287;r~2=0.0461,P=0.1830)。结论:线粒体数目是潜在的肝癌生长标志物。但常用的线粒体定量分子HSP60与COX Ⅳ并不能准确定量肝癌线粒体。
Objective: To study the correlation between the number of mitochondria and the growth of liver cancer. Methods: The number of mitochondria in hepatocellular carcinoma was determined by electron microscopy. The expression of mitochondrial COX Ⅳ and HSP60 in hepatocellular carcinoma was evaluated by immunohistochemistry. The correlation between the quantitative results of mitochondria and the expressions of COX Ⅳ and HSP60 in hepatocellular carcinoma was analyzed Sex, and compared the number of mitochondria in liver cancer, COX Ⅳ and HSP60 expression and the relationship between the diameter of liver cancer. Results: The number of mitochondria (P <0.001) and the expression of COX Ⅳ and HSP60 in hepatocellular carcinoma tissues were significantly lower than those in adjacent tissues (P = 0.0417, P = 0.0290). The expression of COX Ⅳ and HSP60 had no significant correlation with the number of mitochondria (r ~ 2 = 0.009, P = 0.5468; r ~ 2 = 0.056, P = 0.1396). There was a significant negative correlation between the number of mitochondria and tumor diameter (r ~ 2 = 0.1086, P = 0.0434). There was no significant correlation between the expression of COX Ⅳ and HSP60 and tumor diameter (r ~ 2 = 0.0251, P = 0.3287; = 0.1830). Conclusion: The number of mitochondria is a potential marker of liver cancer growth. However, the commonly used mitochondrial quantitative molecules HSP60 and COX Ⅳ do not accurately quantify HCC mitochondria.