2011年从辽宁省丹东地区蚊虫样品中分离到6株病毒,采用逆转录-聚合酶链式扩增(RT-PCR)检测方法,结果黄病毒属通用引物和库蚊黄病毒特异性引物均为阳性。6株病毒核苷酸序列经基因库(GenBank)比对后,证实6株病毒为库蚊黄病毒,为我国首次报道。将病毒NS5基因和E基因核苷酸序列与GenBank中10株库蚊黄病毒参考毒株核苷酸序列进行比较,并构建遗传进化树,结果显示本次分离的6株病毒与美国和日本的毒株亲缘关系较近。 Six strains of viruses were isolated from the mosquito samples in Dandong, Liaoning Province in 2011 by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that all flavivirus-specific and virulent primers were Positive. The nucleotide sequences of six viruses were verified by GenBank. The six viruses were identified as Culex pipiens and were reported for the first time in China. The nucleotide sequences of NS5 gene and E gene were compared with the nucleotide sequences of 10 Culex pipiens strains in GenBank and the phylogenetic tree was constructed. The results showed that the 6 viruses isolated from this strain were identical with those of the U.S. and Japan Relatives of strains closer.