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本文探讨了小鼠肝脂堆积程度的超声影像学半定量检测方法。选取正常C57BL/6J小鼠、高脂饮食诱导的轻度脂肪肝HFD小鼠模型和高热能饮食诱导的中重度脂肪肝KKAy小鼠模型,分别应用肝脏病理学分析和肝组织甘油三酯含量评价动物肝脏的脂质堆积程度。用小动物超声成像系统测定肝组织内与超声波传播同方向的一定距离间2处区域的超声波回声强度,并计算回声衰减系数a值。采用Spearman等级相关分析方法对动物肝脏甘油三酯含量和回声衰减系数a值进行相关性分析。结果显示:与CON组相比,HFD小鼠和KKAy小鼠均具有明显的肝脏脂质堆积,且KKAy小鼠的病变程度较HFD小鼠明显加重。超声波检测结果显示,与CON组相比,HFD小鼠、KKAy小鼠的回声衰减系数a值依次递增。小鼠的肝脏回声衰减系数a值与其肝脏甘油三酯含量具有明显的正相关性,相关系数r=0.744(P<0.01)。本研究表明,肝脏回声衰减系数法可以用来客观评价小鼠肝脏脂质堆积程度。该方法简便易行且具有无创性,可用于监测小鼠肝脏脂肪性病变过程及其药物治疗效果。
This article explores the degree of hepatic lipid accumulation in mice semi-quantitative detection of ultrasound imaging methods. The normal C57BL / 6J mice, HFD mice induced by high-fat diet and KKAy mice model induced by high-calorie diet were used to evaluate the pathological changes of liver and triglyceride Animal liver lipid accumulation level. Ultrasound echo intensity in the liver and liver tissue at two locations in the same direction with the same direction of ultrasonic wave transmission was measured with a small animal ultrasound imaging system and the value of the echo attenuation coefficient a was calculated. Spearman rank correlation analysis was used to analyze the correlation between the hepatic triglyceride content and the echo attenuation coefficient a. The results showed that compared with CON group, both HFD mice and KKAy mice had obvious liver lipid accumulation, and the severity of lesions in KKAy mice was significantly higher than that in HFD mice. Ultrasound results showed that, compared with the CON group, HFD mice, KKAy mouse echo attenuation coefficient a value in increasing order. The mouse liver echo attenuation coefficient a value and liver triglyceride content has a significant positive correlation, the correlation coefficient r = 0.744 (P <0.01). This study shows that the liver echo attenuation coefficient method can be used to objectively evaluate the degree of liver lipid accumulation in mice. The method is simple and easy to operate and has noninvasive properties and can be used for monitoring the fatty liver lesion in mice and the therapeutic effect of the drug.