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目的观察化瘀止痛方及其拆方对人子宫腺肌病细胞缩宫素受体(OTR)mRNA、雌激素受体α(ERα)mRNA表达的影响。方法将化瘀止痛方及其拆方分为化瘀止痛方高、低剂量组,缓急止痛方高、低剂量组,活血行气方高、低剂量组,另设空白对照组、基质组及米非司酮(RU486)组,每组5例,采用胶原酶消化法培养人子宫腺肌病细胞,采用荧光RT-PCR检测药物干预前后子宫腺肌病病灶细胞OTR mRNA、ERαmR-NA表达的变化。结果与空白对照组比较,各给药组子宫腺肌病细胞的OTR mRNA表达均有不同程度下降,化瘀止痛方高、低剂量组及活血行气高剂量组的OTR mRNA表达显著下降,差异均有统计学意义(P<0.05);与RU486组比较,化瘀止痛方高、低剂量组OTR mRNA表达均有不同程度下降,差异均有统计学意义(P<0.05);与缓急止痛低剂量组比较,化瘀止痛方高、低剂量组OTR mRNA表达显著下降,差异有统计学意义(P<0.05)。与空白对照组比较,各给药组细胞的ERαmRNA表达均有不同程度的下降,化瘀止痛方高、低剂量组及缓急止痛高、低剂量组的ERαmRNA表达显著下降,差异有统计学意义(P<0.05)。与RU486对照组比较,化瘀止痛方高、低剂量组、缓急止痛高剂量组子宫腺肌病细胞ERαmRNA表达显著下降,差异有统计学意义(P<0.05)。化瘀止痛方高剂量组与活血行气高剂量组比较,子宫腺肌病细胞ERαmRNA表达显著下降,差异有统计学意义(P<0.05)。结论化瘀止痛方可能通过降低子宫腺肌病病灶细胞OTR mRNA、ERαmRNA表达,减缓子宫平滑肌的收缩强度,从而缓解患者的痛经症状,在降低子宫腺肌病病灶细胞OTR mRNA表达方面活血行气药贡献值较大,在子宫腺肌病病灶细胞ERαmRNA表达变化中缓急止痛药的贡献值较大。
Objective To observe the effects of Huayu Zhitong Recipe and its decomposed formulas on the expression of OTR mRNA and ERα mRNA in human adenomyosis cells. Methods Huayu Zhitong Recipe and its disassembly prescription were divided into high and low dose group of Huayu Zhitong Fang, high dose and low dose group, Mifepristone (RU486) group, 5 cases in each group. Human adenomyosis cells were cultured by collagenase digestion. The expression of OTR mRNA and ERαmR-NA in the cells of adenomyosis before and after drug intervention was detected by fluorescence RT-PCR Variety. Results Compared with the blank control group, the expression of OTR mRNA of the adenomyosis cells in each administration group decreased to different extents. OTR mRNA expression in the high-dose, low-dose and low-dose groups of Huayu Zhitong was significantly decreased, the difference (P <0.05). Compared with RU486 group, the expression of OTR mRNA in high and low dose group of Huayu Zhitong Decoction decreased to some extent, the difference was statistically significant (P <0.05) Compared with the dose group, the expression of OTR mRNA in Huayu Zhitong Fang high dose group and low dose group was significantly decreased, the difference was statistically significant (P <0.05). Compared with the blank control group, the expression of ERαmRNA in each group decreased to different extents. The expression of ERαmRNA in high and low dose group of Huayu Zhitong, high and low dose group was significantly decreased, the difference was statistically significant ( P <0.05). Compared with RU486 control group, ERαmRNA expression of adenomyosis cells in high, low dose group and high-dose group of Euphoria relieving pain were significantly decreased (P <0.05). Compared with high-dose Huoxuzhitong high-dose group and high-dose Huoxuyitong group, the expression of ERαmRNA in adenomyosis cells was significantly decreased (P <0.05). Conclusion Huayu Zhitong Prescription can alleviate the dysmenorrhea symptoms by decreasing the expression of OTR mRNA and ERαmRNA in the cells of adenomyosis and relieve the contractile strength of uterine smooth muscle. Huayu qi medicine can reduce the expression of OTR mRNA in the cells of adenomyosis The contribution value is larger, and the contribution value of the emergency pain killer in the change of the expression of ERαmRNA in the adenomyosis cell of the adenomyosis is larger.