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目的建立一种便捷灵敏的青蒿素含量测定方法,并测定其在不同溶剂中的溶解度及油水分配系数。方法均匀试验设计优化青蒿素的柱前衍生条件,采用HPLC-ECD法进行测定。结果优化的青蒿素柱前衍生条件为:1 mL青蒿素无水乙醇溶液与4 mL氢氧化钠0.01 mol·L-1于70℃水浴中反应50 min后,冷却至室温,再加入5 mL醋酸0.06 mol·L-1。青蒿素在0.0015~0.7500μg范围内线性关系良好。结论建立的柱前衍生HPLC-ECD法专属性强、灵敏度高,适用于青蒿素的测定。
Objective To establish a convenient and sensitive method for the determination of artemisinin, and determine its solubility in different solvents and oil-water partition coefficient. Methods Uniform experimental design was used to optimize the pre-column derivatization conditions of artemisinin. The HPLC-ECD method was used to determine the pre-column derivatization conditions. Results The optimized conditions of pre-column derivatization of artemisinin were as follows: 1 mL of artemisinin absolute ethanol solution was reacted with 4 mL sodium hydroxide 0.01 mol·L-1 in 70 ℃ water bath for 50 min, then cooled to room temperature and then added with 5 mL acetic acid 0.06 mol·L-1. Artemisinin has good linearity in the range of 0.0015 ~ 0.7500μg. Conclusion The pre-column derivatization HPLC-ECD method has the advantages of high specificity and high sensitivity, and is suitable for the determination of artemisinin.