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目的探讨apollon和Caspase-9在人脑胶质瘤组织中的表达及临床意义。方法按Kernohan方法将84例胶质瘤分为Ⅰ级15例,Ⅱ级32例,Ⅲ级23例,Ⅳ级14例;另外75例正常脑组织作为对照组。采用免疫组化SP法检测apollon和Caspase-9的表达。结果胶质瘤组apollon阳性表达率明显高于对照组(77.38%vs.9.33%),而Caspase-9的阳性表达率明显低于对照组(40.47%vs.90.67%)(P<0.05)。随着胶质瘤恶性程度的增高,apollon蛋白的表达逐渐增强,而Caspase-9蛋白的表达逐渐降低;apollon与Caspase-9蛋白表达呈负相关(r=-0.263,P<0.05)。结论 Apollon蛋白的过度表达和Caspase-9蛋白低表达与脑胶质瘤恶性程度相关,并可能作为评价胶质瘤恶性程度生物学指标。
Objective To investigate the expression of apollon and Caspase-9 in human glioma and its clinical significance. Methods According to the method of Kernohan, 84 cases of glioma were classified into Ⅰ grade in 15 cases, Ⅱ grade in 32 cases, Ⅲ grade in 23 cases and Ⅳ grade in 14 cases. Another 75 cases of normal brain tissue served as the control group. Immunohistochemical SP method was used to detect the expression of apollon and Caspase-9. Results The positive expression of apollon in glioma group was significantly higher than that in control group (77.38% vs.9.33%), while the positive expression rate of Caspase-9 in glioma group was significantly lower than that in control group (40.47% vs.90.67%, P <0.05). With the increase of the malignant degree of glioma, the expression of apollon protein gradually increased and the expression of Caspase-9 protein gradually decreased. The expression of apollon and Caspase-9 protein was negatively correlated (r = -0.263, P <0.05). Conclusion The overexpression of Apollon and the low expression of Caspase-9 are associated with the malignant degree of glioma and may be used as biomarkers to evaluate the malignant degree of glioma.