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目的:评价7，8-二羟基黄酮（7，8-dihydroxyflavone, 7，8-DHF）对过氧化氢（hydrogen peroxide, Hn 2On 2）引起PC12细胞损伤的影响，并探究其机制。n 方法:采用随机数字表法将PC12细胞分为4组（每组4皿）：对照组（C组）、7，8-DHF组（D组）、Hn 2On 2组（H组）、7，8-DHF+Hn 2On 2组（D+H组）。C组加入PBS缓冲液，D组加入25 μmol/L 7，8-DHF，H组和D+H组均加入200 μmol/L Hn 2On 2，D+H组在加入Hn 2On 2前采用25 μmol/L 7，8-DHF预处理1 h。各组细胞孵育24 h后采用细胞计数试剂盒（cell counting kit-8，CCK-8）法检测细胞活力，2，4-二硝基苯肼显色法检测乳酸脱氢酶（lactate dehydrogenase, LDH）释放率，Hoechst染色法和膜联蛋白V-异硫氰酸荧光素（Annexin V-fluorescein isothiocyanate, FITC）/碘化丙啶（propidium iodide, PI）双染法结合流式细胞仪观察并分析细胞凋亡情况，实时荧光定量PCR（real-time quantitative PCR, RT-qPCR）法测定酸敏感离子通道3（acid-sensing ion channel 3, ASIC3）、B淋巴细胞瘤-2（B-cell lymphoma-2, Bcl-2）/B淋巴细胞瘤-2相关X蛋白（B-cell lymphoma-2 related X protein, Bax）的mRNA表达，Western blot法测定ASIC3、Bcl-2/Bax、活化的含半胱氨酸的天冬氨酸蛋白酶-3（cleaved cysteinyl aspartate specific proteinase-3, cleaved caspase-3）蛋白水平。n 结果:与C组比较：H组PC12细胞数量和细胞活力明显降低，LDH释放率及细胞凋亡率明显升高，ASIC3 mRNA表达和蛋白水平均上调，cleaved caspase-3蛋白水平上调，Bcl-2/Bax mRNA比值和蛋白比值均降低（n P0.05）。与H组比较，D+H组细胞形态接近正常，细胞数量和细胞活力明显升高，LDH释放率及细胞凋亡率明显降低，ASIC3 mRNA表达和蛋白水平均下调，cleaved caspase-3蛋白表达下调，Bcl-2/Bax mRNA比值和蛋白比值均升高（n P<0.05）。n 结论:7，8-DHF可以减轻Hn 2On 2引起的PC12细胞损伤，其机制可能与抑制ASIC3信号从而减轻细胞凋亡有关。n “,”Objective:To evaluate the effects of 7, 8-dihydroxyflavone (7, 8-DHF) on PC12 cell damage induced by hydrogen peroxide (Hn 2On 2) and explore its related mechanisms.n Methods:According to the random number table method, PC12 cells were divided into four groups (n n=4): a control group (group C), a 7, 8-DHF group (group D), a Hn 2On 2 group (group H), and a 7, 8-DHF+Hn 2On 2 group (group D+H). PBS solution was added in group C, 25 μmol/L 7, 8-DHF was added in group D and 200 μmol/L H n 2On 2 was added in groups H and D+H. Furthermore, group D+H was treated with 25 μmol/L 7, 8-DHF for 1 h before the addition of H n 2On 2. After 24 h of incubation, cell viability was detected by cell counting kit-8 (CCK-8) assay in each group. The release rate of lactate dehydrogenase (LDH) was determined by 2,4-dinitrophenylhydrazine colorimetry. Apoptosis was observed and analyzed by Hoechst staining and Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining combined with flow cytometry. The expression of acid-sensing ion channel 3 (ASIC3) and B-cell lymphoma-2 (Bcl-2)/B-cell lymphoma-2 related X protein (Bax) mRNA were detected by real-time quantitative PCR (RT-qPCR). The expression of ASIC3, Bcl-2/Bax and cleaved cysteinyl aspartate specific proteinase-3 (cleaved caspase-3) were detected by Western blot.n Results:Compared with group C, group H presented remarkably decreases in the number of PC12 cells and cell viability; significant increases in LDH release rate and apoptosis rate, ASIC3 mRNA and protein expression, the expression of cleaved caspase-3 protein; and remarkably decreases in Bcl-2/Bax mRNA ratio and protein ratio (n P0.05). Compared with group H, group D+H showed cell morphology that was close to the normal; significant increases in the number of cells and cell viability; remarkable decreases in LDH release rate and apoptosis rate, ASIC3 mRNA and protein expression, and cleaved caspase-3 protein expression; and significant increases in Bcl-2/Bax mRNA ratio and protein ratio (n P<0.05).n Conclusions:7, 8-DHF can relieve PC12 cell damage induced by Hn 2On 2 which may be related to reduced apoptosis due to inhibition of ASIC3 signals.n