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Purpose: To identify differential genes expressed in the rabbit ciliary epithelium duringthe circadian cycle of aqueous flow.Methods: Total RNA from ciliary epithelium of rabbits at 8AM (light on 1 hour) and8PM(light off 1 hour) were compared by differential display reverse transcription-polymerase chain reaction(DD RT-PCR), using 6 % denaturing polyacrylamide electro-phoresis, choose differential display bands, cut and reamplify with the same primer, cloneand sequence. Search the database of Genbank, prolong them with 5’ RACE and 3’RACE technique then clone, sequence and search database of Genbank.Results: 93 Significant differences gene expression were detected between light on andlight off in the rabbit ciliary epithelium.Conclusion: Differential display is a powerful tool to screen differentially expressedgenes in circadian rhythm of ciliary epithelium. Eye Science 2001; 17:133 ~137.
Purpose: To identify differential genes expressed in the rabbit ciliary epithelium during the circadian cycle of aqueous flow. Methods: Total RNA from ciliary epithelium of rabbits at 8AM (light on 1 hour) and 8PM (light off 1 hour) were compared by differential display reverse transcription -polymerase chain reaction (DD RT-PCR) using 6% denaturing polyacrylamide electro-phoresis, choose differential display bands, cut and remplify with the same primer, clone and sequence. Search the database of Genbank, prolong them with 5 ’RACE and 3 ’RACE technique then clone, sequence and search database of Genbank. Results: 93 Significant differences gene expression were detected between light on and light off in the rabbit ciliary epithelium. Contact: Differential display is a for tool to screen differentially expressed genes in circadian rhythm of ciliary Eye Science 2001; 17: 133-137.