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目的:研究自制几丁糖-胶原膜在上颌窦提升牙种植中的引导骨再生效果,探讨成骨细胞藻酸盐凝胶作为植骨材料的潜力。方法:6条犬拔除双侧上颌第4前磨牙及第1磨牙,2月后取髂骨培养成骨细胞。3月后行双侧上颌窦底提升术,每侧植入2枚种植体,上颌窦底及颊侧骨缺损植入藻酸盐-成骨细胞凝胶,左侧覆盖膜作为实验组,右侧不盖膜作为对照组。术后2、4、6月各处死2条犬,X线及免疫组化法观察上颌窦底及颊侧骨缺损区骨再生。结果:实验组种植体根部及上颌窦底有大量骨再生,种植体发生骨结合;对照组只有少量骨再生;免疫组化显示植入藻酸盐-细胞凝胶2、4月时,所有种植体根部的骨缺损区,新生骨反应呈阳性。讨论:几丁糖-胶原膜可以屏障纤维组织,保护骨源性细胞在藻酸盐细胞凝胶支架中生长分化,使骨缺损再生。结论:上颌窦底提升术中植入成骨细胞藻酸盐凝胶并覆盖自制几丁糖-胶原膜,可引导上颌窦底骨再生,其中藻酸盐细胞凝胶起支架作用,膜起屏障作用。
OBJECTIVE: To study the effect of self-made chitosan-collagen membrane on guide bone regeneration in maxillary sinus ascending dental implants and to explore the potential of osteoblast alginate gel as a bone graft material. Methods: Six dogs were removed bilateral maxillary fourth premolar and first molars. Two months later, ilium was used to culture osteoblasts. In March, bilateral maxillofacial ascending surgery was performed. Two implants were implanted on each side. Alginate-osteoblastic gel was implanted on the maxillary sinus floor and the buccal bone defect. The left cover membrane was used as the experimental group and the right Side of the membrane as a control group. Two dogs were sacrificed at 2, 4 and 6 months after operation, and bone regeneration was observed by X-ray and immunohistochemistry in the maxillary sinus floor and buccal bone defect. RESULTS: In the experimental group, there was a large amount of bone regeneration at the root and the maxillary sinus floor, and the implant was bone-bonded. In the control group, only a small amount of bone was regenerated. Immunohistochemistry showed that when all alginate-cell gels were implanted in February and April, Bone root defect area, new bone reaction was positive. Discussion: Chitosan - collagen membrane can barrier the fibrous tissue, protect bone cells in alginate gel cell growth and differentiation, the regeneration of bone defects. CONCLUSIONS: Osteoblasts alginate gel implanted into maxillary sinus floor elevation and covered with homemade chitosan-collagen membrane can lead to the regeneration of maxillary sinus bone. Alginate gel acts as a scaffold and membrane barrier effect.