【摘 要】
：
Membrane-type 1 matrix metalloproteinase (MT1 MMP/MMP 14) plays crucial roles in tumor cell growth, invasion, and angiogenesis. To clarify whether the endogenou
【机 构】
：
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College of Huazhong Univers
论文部分内容阅读
Membrane-type 1 matrix metalloproteinase (MT1 MMP/MMP 14) plays crucial roles in tumor cell growth, invasion, and angiogenesis. To clarify whether the endogenously expressed MT1-MMP in metastatic human ovarian carcinoma cell lines SKOV3 plays a critical role in tumor cell invasiveness, antisense MT1-MMP cloned in eukaryotic expression vector pMMP14as was transferred into SKOV3 cells. 48h after transfection, decreased expression of endogenous MT1-MMP protein was detected in pMMP14as transfected SKOV3 cells and the activation of pro MMP2was inhibited markedly. The mean percentage of invasive cells was (62. 50 ±5. 30) % in pMMP14as-transfected cells, which was obviously less than that (97.20±6.90) % in the control.Thus, antisense MT1 MMP effectively inhibited the endogenous MT1 MMP expression and the invasiveness in SKOV3 cells, suggesting that MT1-MMP may be a therapeutic target molecule for human invasive ovarian cancers.
其他文献
压疮是由于局部软组织持续受压引起血液动力学改变,导致组织细胞缺血缺氧、营养代谢障碍而发生变性坏死的病理过程,它是临床常见的并发症之一,并且容易引起感染,一旦恶化会给
在急诊科机动车碰撞是患者肺部损伤的最常见的原因.人为锤击胸部所致的肺部实质损伤则较少见,在临床治疗上极易被轻忽视.本文以临床工作中遇到的人为锤击胸部致肺出血休克1例
The heat shock proteins (HSPs) 70 and HSP 27 expression in patients with non-small cell lung cancer (NSCLC) was studied and the relationship between HSP 70 and
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生、测量监控等方面人手,介绍了S226海滨大桥
以小麦胚芽为原料,首先进行脱脂处理,从脱脂胚中分离出清蛋白后再提取出其中的球蛋白,对影响球蛋白提取工艺的参数,如盐浓度、pH等进行单因素研究,并对氮的去向进行了测定分
To investigate the effect of propofol on the release of glutamate and γ-aminobutyric acid (GABA) from rat hippocampal synatosomes, synaptosomes was made from h
To study the effects of Icariin on expression of osteopontin (OPN) mRNA and type Ⅰ collagen in rat osteoblasts in vitro and to explore its possible mechanisms
麻醉恢复室又称为麻醉后监测治疗室(postanesthesia care unit,PACU),是对麻醉后患者进行严密观察、监测、继续治疗直至患者的生命体征恢复稳定的科室.术后患者由于受麻醉、