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利用SDS聚丙烯酰胺凝胶电泳,分析了早熟大豆品种晋大75和晚熟大豆品种晋大53在开花后不同时期球蛋白亚基积累的变化以及其与品质的关系。结果表明:不同亚基的积累规律不同,在合成积累的时间顺序上存在差异,早熟、晚熟品种水溶蛋白和盐溶蛋白的低分子量蛋白亚基(B、C、D区)在开花后15 d开始逐渐合成,早熟品种的高分子量球蛋白亚基(A′、A区)在开花后15 d开始合成,比晚熟品种提前20~25 d。晋大75水溶蛋白的剧增期在开花50~65 d,而盐溶蛋白的剧增期在开花后30~35 d和50~65 d;晋大53水溶蛋白的剧增期为开花后25~30 d和70~75 d,盐溶蛋白的剧增期在开花后30~35 d和50~65 d。
Using SDS polyacrylamide gel electrophoresis, we analyzed the changes of globulin subunits accumulation in early maturity soybean Jinnai 75 and late maturity soybean Jinda 53 and their relationship with quality. The results showed that the accumulation of different subunits was different, and there were differences in the time sequence of the accumulation. The low molecular weight protein subunits (B, C and D) of the early maturing and late maturing varieties of water soluble protein and salt soluble protein appeared at 15 d after flowering The high molecular weight globulin subunits (A ’, A region) of early maturing varieties began to be synthesized gradually 15 d after anthesis, 20 ~ 25 d earlier than the late maturing varieties. The increasing stage of Jinda 75 water-soluble protein was between 50 and 65 days after flowering, while the salinization period of salt-soluble protein was between 30 and 35 d after flowering and from 50 to 65 days after flowering. The sharp increase period of Jinda 53 water-soluble protein was 25 ~ 30 d and 70 ~ 75 d, the sharp increase of salin during 30 ~ 35 d and 50 ~ 65 d after anthesis.