论文部分内容阅读
目的:研究RhD抗原阳性(变异)、产生IgG-D抗体致新生儿溶血病个体的RHD基因结构。方法:采用常规血清学方法,检测1例RhD抗原,鉴定个体体内引发新生儿溶血病抗体的性质。采用PCR-SSP方法检测分析RHD/RHCE基因。结果:RhD抗原检测符合部分D类,体内存在IgG-D抗体。基因分析发现RHD与RHCE基因交换形成一种新的RHD-CE(3~6)-D融合基因。RHD杂合性试验显示为RHD+/RHD-。结论:该个体Rh血型为RH DVIⅢ型(部分D类)。
OBJECTIVE: To study the RHD gene structure of RhD antigen-positive (mutated) and IgG-D antibody-producing neonates with hemolytic disease. Methods: One case of RhD antigen was detected by routine serological methods to identify the properties of the antibodies that cause hemolytic disease in neonates. RHD / RHCE gene was detected by PCR-SSP method. Results: The detection of RhD antigen was in accordance with part D, and IgG-D antibody was present in the body. Gene analysis found RHD and RHCE gene exchange to form a new RHD-CE (3 ~ 6) -D fusion gene. The RHD heterozygosity test is shown as RHD + / RHD-. Conclusion: The individual Rh blood type is RH DVI type III (part D).