2型糖尿病及并发肾病模型大鼠肠道菌群多样性分析

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为分析2型糖尿病及并发肾病大鼠模型肠道菌群失衡模式及其与代谢和炎症相关指标之间的关系,通过高糖高脂饲料喂养联合腹腔注射链脲佐菌素法构建2型糖尿病大鼠模型,用相同方法联合单肾切除构建糖尿病肾病大鼠模型.成模后采集血液、尿液标本用于检测分析相关代谢及炎症水平变化,采集结肠粪便标本进行高通量测序分析.结果表明,与对照组相比,两模型组大鼠发生明显的胰岛素抵抗和代谢异常,血循环中均存在低程度炎症,且糖尿病肾病组更为显著;两模型组大鼠肠道菌群多样性较对照组显著增加(P<0.01),但物种丰度在3个组间无显著差异;两模型组肠道中Romboutsia丰度显著低于对照组,毛螺菌科(Lachnospiraceae)和瘤胃球菌科(Ruminococcaceae)的丰度显著高于对照组(P<0.05);埃希氏-志贺氏菌属(Escherichia-Shigella)、Ruminococcus和Faecalibaculum在肾病组富集(P<0.05).结果提示,2型糖尿病及其并发肾病大鼠肠道菌群结构均向有害菌偏移,糖尿病肾病组发生的改变更具致病性且其进展可能与肠道内特定有益菌和有害菌相对丰度变化所参与的炎症反应有关.
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