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目的:探讨不同标准对荧光原位杂交技术(fluorescence in situ hybridization,FISH)诊断膀胱癌的敏感度和特异性的影响。方法:选择20例健康人为正常组,计算FISH检查正常阈值;选择143例血尿患者为病例组.经FISH检查、尿脱落细胞学检查,比较Urovysion膀胱癌探针试剂盒标准和正常阈值标准诊断膀胱癌的敏感度和特异性。结果:采用Urovysion标准和正常阈值标准对膀胱癌的诊断敏感度分别为73.1%和100%,均较尿脱落细胞学检查明显增高,三者对膀胱癌诊断的特异性分别为90.0%、86%和100%。Urovysion标准与尿脱落细胞学联合检查时对膀胱癌诊断的敏感度明显升高,差异具有统计学意义(P<0.01)。结论:FISH检查较尿脱落细胞学检查诊断膀胱癌的敏感度显著提高,相比Urovysion标准,正常阈值更适合FISH诊断膀胱癌的标准。FISH与尿脱落细胞学联合检查能显著提高膀胱癌的诊断敏感度。
Objective: To investigate the effect of different standards on the sensitivity and specificity of fluorescence in situ hybridization (FISH) in the diagnosis of bladder cancer. Methods: Twenty healthy subjects were selected as the normal group, normal threshold of FISH was calculated, 143 cases of hematuria were selected as case group, FISH and urinary cytology were performed to compare urinary bladder cancer with Urovysion bladder cancer probe kit standard and normal threshold Cancer sensitivity and specificity. Results: The diagnostic sensitivity of Urovysion standard and normal threshold to bladder cancer were 73.1% and 100%, respectively, which were significantly higher than that of urine cytology. The diagnostic specificity of bladder cancer was 90.0% and 86% respectively And 100%. Urovysion criteria and urinary exfoliative cytology combined diagnosis of bladder cancer significantly increased the sensitivity of the test, the difference was statistically significant (P <0.01). CONCLUSION: The sensitivity of FISH to diagnosing bladder cancer is significantly higher than that of urine cytology. Compared with Urovysion criterion, the normal threshold is more suitable for FISH diagnosis of bladder cancer. FISH combined with exfoliative cytology can significantly improve the diagnostic sensitivity of bladder cancer.