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目的研究甲基丙烯酸环氧丙酯(GMA)对人支气管上皮(16HBE)细胞染色体损伤的情况,探讨其潜在致癌性。方法 GMA染毒16HBE细胞后,收获不同染毒剂量(4、8、12、16、20μg/ml)、染毒次数(1、2、3次)、转化代数(10、30代)的细胞进行染色体畸变分析。结果在4～20μg/ml剂量范围内,随染毒剂量的增加,染色体畸变率(3%、6%、7%、11%、14%)升高,并呈剂量-反应关系;随着染毒次数增加,3次染毒的细胞染色体畸变率(6%、7%、10%)显著增高;转化第10代细胞表现为染色体结构畸变,而转化第30代细胞主要表现为染色体数目畸变。结论 GMA能够诱导16HBE细胞染色体畸变,且畸变类型由早期的结构畸变转变为数目畸变。
Objective To investigate the chromosomal damage of human bronchial epithelial (16HBE) cells by glycidyl methacrylate (GMA) and to investigate the potential carcinogenicity. Methods After 16HBE cells were treated with GMA, cells were harvested at different doses (4, 8, 12, 16 and 20μg / ml) Chromosome aberration analysis. Results The rate of chromosome aberration (3%, 6%, 7%, 11%, 14%) increased with dose increasing in the range of 4 ~ 20μg / ml with a dose-response relationship. Toxicity increased, the chromosome aberration rate (6%, 7%, 10%) was significantly increased after 3 times of exposure. The 10th generation of transformed cells showed chromosome structural aberration, while the 30th generation of transformed cells mainly showed chromosome number distortion. Conclusion GMA can induce chromosomal aberration in 16HBE cells and the type of aberrations changes from early structural aberrations to numerical aberrations.