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目的:探讨甲基苯丙胺的神经毒性及噻萘普汀的保护机制。方法:设立对照组(b)和实验组(a、c),a组腹腔注射甲基苯丙胺;c组在予甲基苯丙胺前7天予噻萘普汀;b组注射等体积生理盐水。实验后采用苏木精-伊红染色,光镜观察;TUNEL法检测凋亡。结果:甲基苯丙胺可损害神经元的轴突、树突,并诱导凋亡。结论:甲基苯丙胺通过诱导凋亡,引起神经毒性;预先予噻萘普汀具保护效应。
Objective: To investigate the neurotoxicity of methamphetamine and the protective mechanism of thiabeptin. Methods: The control group (b) and experimental group (a, c) were established. Group a received intraperitoneal injection of methamphetamine; group c received thiapeptin 7 days prior to methamphetamine; group b received an equal volume of normal saline. After the experiment, hematoxylin-eosin staining and light microscopy were performed. Apoptosis was detected by TUNEL assay. Results: Methamphetamine can impair neuronal axons, dendrites, and induce apoptosis. Conclusion: Methamphetamine can induce neurotoxicity by inducing apoptosis. Pretreatment with thiamprenol has protective effect.