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将7周龄棉鼠的尾巴割破,通过已感染棉鼠丝虫的柏氏禽刺螨叮咬使棉鼠感染,100天后从鼠的胸腔中取出雌雄成虫,放入-20℃PH为7.2的磷酸缓冲盐水溶液中(PBS),在室温下融化后放入Potter-Elvejham匀浆器内加以同一冻融的PBS,研磨20分钟并经机械震荡5分钟后按每份0.5ml分装,冰冻至-20℃,测定其蛋白质浓度为10.1mg/ml。经再次融化后加福氏完全佐剂(1:1.5)乳化。每只棉鼠按每公斤体重10mg的蛋白质量于鼠的腿部肌注。以2周龄和14周龄的鼠作预防接种试验,每个试验组用鼠7～10只,第一次注射后隔2周注射第2次,再隔4周注射
The tail of a 7-week-old cotton rat was cut and the cotton rat was infected through a bite of Platycodon pylori infected with a silk fibroin-infected mouse. After 100 days, the adult male and female were removed from the rat’s chest and put into a phosphate solution Buffer saline (PBS), thawed at room temperature, placed in a Potter-Elvejham homogenizer with the same freeze-thawed PBS, ground for 20 minutes and mechanically shaken for 5 minutes and then aliquoted in 0.5 ml portions, frozen to - 20 ℃, the determination of its protein concentration of 10.1mg / ml. After re-thawing, Corfu’s complete adjuvant (1: 1.5) emulsified. Each cotton rat protein mass per kilogram of body weight of 10mg in the mouse leg intramuscular injection. Rats were vaccinated at 2 and 14 weeks of age with 7 to 10 mice in each group. The rats were injected 2 times at 2 weeks after the first injection and 4 weeks after the first injection