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目的：研究内毒素血症大鼠血中促炎性细胞因子白细胞介素 ６（ Ｉ Ｌ ６）、肿瘤坏死因子 α（ Ｔ Ｎ Ｆ α）及花生四烯酸代谢产物前列腺素 Ｅ２ （ Ｐ Ｇ Ｅ２）的含量和血中分泌型磷脂酶 Ａ２ （ｓ Ｐ Ｌ Ａ２）活性产生的动态变化规律，探讨脂多糖（ Ｌ Ｐ Ｓ）全面启动机体炎症反应的机制。方法：采用大鼠腹腔注射 Ｌ Ｐ Ｓ复制大鼠急性内毒素血症模型，分别动态测定了正常大鼠和急性内毒素血症大鼠血清中 Ｉ Ｌ ６、 Ｔ Ｎ Ｆ α、 Ｐ Ｇ Ｅ２ 含量及ｓ Ｐ Ｌ Ａ２ 活性。结果： Ｌ Ｐ Ｓ注射后，大鼠血清中 Ｉ Ｌ ６、 Ｔ Ｎ Ｆ α、 Ｐ Ｇ Ｅ２ 含量及ｓ Ｐ Ｌ Ａ２ 活性均显著升高（与正常组比较 Ｐ＜ ００５ 或 Ｐ＜００１ ）， Ｔ Ｎ Ｆ α最先升高，在 ４５ 分钟达峰值并呈双相变化，ｓ Ｐ Ｌ Ａ２ 和 Ｐ Ｇ Ｅ２ 达峰值时间（１５ 小时）晚于 Ｔ Ｎ Ｆ α，并分别呈双相及三相变化； Ｉ Ｌ ６ 缓慢升高于 ６ 小时达峰值并呈单相变化。相关性分析显示， Ｔ Ｎ Ｆ α含量升高与ｓ Ｐ Ｌ Ａ２ 活性升高呈正相关（ｒ＝ ０８１９ ６， Ｐ＜ ００５）。结论： Ｌ Ｐ Ｓ可明显引发机体内的早期细胞因子反应，通过迅速激活ｓ Ｐ Ｌ Ａ２ ，促进花生四烯酸代谢，使脂类介质产生增加，可?
Objective: To study the effects of endotoxemia on blood levels of proinflammatory cytokines interleukin 6 (IL 6), tumor necrosis factor α (T N F α) and arachidonic acid metabolites prostaglandin E2 (P G E2 ), And the activity of secreted phospholipase A2 (sPLA2), and to explore the mechanism by which lipopolysaccharide (LPS) can fully activate the inflammatory response in the body. Methods: A rat model of acute endotoxemia was induced by intraperitoneal injection of LPS in rats. The levels of IL-6, T N F α and P G E2 in serum of rats and acute endotoxemia rats were measured dynamically And s P L A2 activity. Results: After LPS injection, the content of IL-6, T Fα, P G E2 and the activity of s P L A2 were significantly increased in rats (P <005 or P <00 01), T N F α firstly increased and peaked at 45 minutes with a biphasic change. The peak time of s P L A2 and P G E2 (1.5 hours) was later than that of T N F α, respectively Biphasic and three-phase changes; I L 6 increased slowly to peak at 6 hours and showed a single phase change. Correlation analysis showed that the increase of T N F α was positively correlated with the increase of s P L A2 activity (r = 0819 6, P <005). CONCLUSION: L P S can obviously induce the early cytokine response in vivo. It promotes the metabolism of arachidonic acid through the rapid activation of s P L A2 and increases the production of lipid media.