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目的探讨雌二醇(E2)和孕酮(P4)对体外培养的人子宫内膜基质细胞(ES细胞)血小板反应素-1(TSP-1)表达的影响。方法 2007年12月至2008年10月在北京协和医院用不同浓度的E2及P4处理体外培养的ES细胞一定时间(研究组),提取细胞RNA和蛋白,分别用Northern blot和Western blot方法比较研究组和未用激素刺激的对照组之间TSP-1 mRNA和蛋白水平的差异。结果 E2能抑制ES细胞TSP-1的表达,与对照组相比,用10nmol/L的E2处理ES细胞后,其TSP-1 mRNA和蛋白水平分别下降(47.6±6.5)%(P<0.05)和(49.0±8.6)%(P<0.05);高浓度P4(10μmol/L)能诱导ES细胞表达TSP-1,与对照组相比,10μmol/L的P4处理ES细胞后,其TSP-1 mRNA和蛋白水平分别是对照组的(2.1±0.4)倍(P<0.05)和(2.3±0.6)倍(P<0.05);低浓度(10nmol/L)P4能增强E2对TSP-1蛋白表达的抑制作用,但对其mRNA的表达无明显影响。结论雌激素和孕激素能有差别地调节ES细胞TSP-1的表达,这一发现为子宫内膜异位症的雌激素依赖提供了证据,同时也部分解释了孕激素治疗子宫内膜异位症的机制。
Objective To investigate the effects of estradiol (E2) and progesterone (P4) on the expression of TSP-1 in cultured human endometrial stromal cells (ES cells). Methods From December 2007 to October 2008, cells treated with different concentrations of E2 and P4 were treated with Peking Union Medical College Hospital for a certain period of time (study group), RNA and protein were extracted and compared with Northern blot and Western blot respectively Differences in TSP-1 mRNA and protein levels between groups and control groups not stimulated with hormones. Results E2 inhibited the expression of TSP-1 in ES cells. Compared with the control group, the TSP-1 mRNA and protein levels of ES cells decreased (47.6 ± 6.5)% (P <0.05) by 10 nmol / And (49.0 ± 8.6)%, respectively (P <0.05). Compared with the control group, ES cells could express TSP-1 by high concentration of P4 (10μmol / L) (P <0.05) and (2.3 ± 0.6) folds of the control group (P <0.05). Low concentrations of P4 (10 nmol / L) increased the expression of TSP-1 The inhibition of its mRNA expression, but no significant effect. Conclusion Estrogen and progestin can differentially regulate the expression of TSP-1 in ES cells. This finding provides evidence for the estrogen dependence of endometriosis and partially explains the effect of progestin on endometriosis Symptoms of the disease.