论文部分内容阅读
采用薄层扫描法测定了刺五加叶中皂苷配基之一齐墩果酸，对刺五加叶中皂苷的水解条件、皂甙配基的薄层层析条件及显色条件进行了研究。确定了用５％Ｈ２ＳＯ４乙醇水（１∶１）溶液，回流１０ｈ，在予制高效硅胶薄层层析板，以苯—丙酮（３６∶１３）为展开剂展开，喷１０％Ｈ２ＳＯ４水溶液，１０５℃烘烤１５ｍｉｎ显色条件，测定了刺五加叶总皂苷中齐墩果酸的含量。本方法的平均回收率９８％，相对标准偏差为３．０％。
The oleanolic acid, one of the saponins in leaves of Acanthopanax senticosus, was determined by thin-layer scanning method. The hydrolysis conditions of saponin in Acanthopanax senticosus leaves, the thin layer chromatography conditions of saponin ligand and the conditions for color development were studied. Determined with 5% H2SO4 ethanol water (1: 1) solution, refluxing 10h, pre-made high-performance silica gel thin layer chromatography plate, benzene - acetone (36:13) as a developing agent, spray 10% H2SO4 aqueous solution, 105 The condition of coloration was determined by baking at 15 °C for 15 min. The content of oleanolic acid in the total saponins of Acanthopanax senticosus was determined. The average recovery of this method is 98% with a relative standard deviation of 3.0%.