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目的探讨醒脑启智胶囊对小鼠脑组织海马区乙酰胆碱酯酶的影响。方法120只昆明种小鼠随机分为假手术组、模型组、醒脑启智胶囊高剂量组、醒脑启智胶囊低剂量组、银杏叶组、尼莫地平组,每组20只,采用双侧颈总动脉结扎反复脑缺血再灌注的方法制备血管性痴呆小鼠模型。术后7d进行行为学实验和脑组织海马区的病理形态学观察,并用原子分光光度法测脑组织海马区乙酰胆碱酯酶活力。结果①行为学实验显示,模型组小鼠学习成绩与记忆成绩下降,各治疗组小鼠的学习成绩与记忆成绩均有提高(P<0·05或P<0·01)。②光镜下病理形态学显示,术后模型组小鼠脑组织海马区呈缺血性病理改变,各治疗组小鼠病变轻于模型组。③模型组小鼠脑组织乙酰胆碱酯酶明显低于假手术组(P<0·01),各用药组均高于模型组(P<0·05或P<0·01)。结论醒脑启智胶囊对血管性痴呆小鼠有明显治疗作用,其作用机理之一是调节脑组织乙酰胆碱酯酶的活性,减轻缺血再灌注损伤。
Objective To investigate the effect of Xingnaoqizhi Capsule on acetylcholinesterase in hippocampus of mice. Methods One hundred and twenty Kunming mice were randomly divided into sham operation group, model group, high-dose group of Xingnaoqizhi capsule, low-dose group of Xingnaoqizhi capsule, Ginkgo biloba group, and nimodipine group. Preparation of vascular dementia mice model by bilateral cerebral ischemia reperfusion with bilateral common carotid artery ligation. At 7 days after operation, behavioral experiments and pathological observations of brain tissue in the hippocampus were performed, and the acetylcholinesterase activity in hippocampus of brain tissue was measured by atomic spectrophotometry. Results 1Behavioral experiments showed that the learning and memory performance of mice in the model group decreased, and the learning and memory performance of the mice in each treatment group increased (P<0.05 or P<0.01). 2 Pathological morphology under light microscope showed that the hippocampus of the mouse model in the postoperative model group showed ischemic pathological changes, and the mice in each treatment group had lighter lesions than the model group. 3Acetylcholinesterase in the mouse model group was significantly lower than that in the sham group (P<0.01), and each group was higher than the model group (P<0.05 or P<0.01). Conclusion Xingnaoqizhi Capsule has obvious therapeutic effect on vascular dementia mice. One of its mechanisms is to regulate the activity of acetylcholinesterase in brain tissue and reduce ischemia-reperfusion injury.