论文部分内容阅读
以灵芝液体发酵菌丝量和胞外多糖含量为评价指标,确定灵芝菌液体发酵最优培养基配方,分析了菌丝体和发酵液中的活性成分。结果表明,灵芝液体发酵最优配方为黄豆粉添加量1.63%,葡萄糖添加量2.33%,可溶性淀粉添加量3%,磷酸二氢钾添加量0.1%,硫酸镁添加量0.05%,VB_1添加量50 mg/L,菌丝体干质量最高达1.94 g/100 mL;发酵液中的胞外多糖质量浓度为431 mg/100 mL;菌丝体胞内多糖质量分数达15.37%,总三萜质量分数达1.36%,蛋白质质量分数为22.93%,多酚质量分数为1.91%。
The optimum fermentation medium for Ganoderma lucidum liquid fermentation was determined with the amount of mycelium of Ganoderma lucidum and the content of extracellular polysaccharide. The active components in mycelium and fermentation broth were analyzed. The results showed that the best formula of liquid fermentation of Ganoderma lucidum was 1.63% of soybean powder, 2.33% of glucose, 3% of soluble starch, 0.1% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate and 50% mg / L, the highest mycelial dry weight was 1.94 g / 100 mL; the concentration of exopolysaccharide in the fermentation broth was 431 mg / 100 mL; the content of intracellular polysaccharide in mycelium reached 15.37%; the total triterpene mass fraction Up to 1.36%, the protein content was 22.93% and the polyphenol content was 1.91%.