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1.引言生物高分子试剂应包括蛋白质、酶、糖等。这些试剂在生化研究和临床诊断上的应用范围很广。它们能测定的物质非常多,对其进行系统分类和整理并不容易。本文就免疫测定法及与之有关试剂的性质、制备及使用方法等加以叙述。 2.抗体(抗血清) 抗体是免疫测定中必备的试剂。这里所说的抗体,指的是免疫球蛋白中的IgG,其分子量约为160000(图1)。制备方法大致有两种。
1. Introduction Biological polymer reagents should include protein, enzymes, sugar and so on. These reagents in biochemical research and clinical diagnosis of a wide range of applications. They can determine the material is very large, its systematic classification and finishing is not easy. This article describes the immunoassay and related reagents, preparation and use of such methods are described. Antibodies (antisera) Antibodies are essential reagents for immunoassays. Antibody, as used herein, refers to IgG in immunoglobulins with a molecular weight of approximately 160,000 (Figure 1). There are basically two preparation methods.