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目的考察秦皮香豆素在不同实验条件下的提取与电泳行为,建立其中主要活性成分秦皮苷、秦皮甲素、秦皮乙素的毛细管区带电泳快速定量方法。方法采用50mmol.L-1硼砂溶液为运行缓冲溶液,熔融石英毛细管有效长度45cm(75μm×53.5cm),以对羟基苯甲酸丙酯为内标,操作电压18kV,柱温25℃,于210nm波长处测定。结果在优化的电泳条件下,3种秦皮香豆素在10min内完全分离。秦皮苷、秦皮甲素、秦皮乙素的线性范围分别为:6.0～96mg.L-1(r=0.9997,n=5),7～112mg.L-1(r=0.9996,n=5)和4.7～75.2mg.L-1(r=0.9994,n=5);平均加样回收率分别为101.74%,l00.92%和102.44%,相应的RSD分别为2.18%,1.97%,2.66%(n=9)。结论本实验为秦皮苷、秦皮甲素和秦皮乙素的同时定量提供了一种快速简便的检测方法。
OBJECTIVE To investigate the extraction and electrophoresis behavior of pectin coumarin under different experimental conditions and to establish a rapid and quantitative method for the determination of the major active components of them, namely epeptidin, aesculin and aescine by capillary zone electrophoresis. Methods A 50 mmol. L-1 borax solution was used as the running buffer solution. The effective length of the fused silica capillary was 45 cm (75 μm × 53.5 cm) with propyl p-hydroxybenzoate as internal standard. The operating voltage was 18 kV and the column temperature was 25 ° C. Department of determination. Results Under the optimized electrophoresis conditions, the three kinds of Qin coumarin completely separated in 10min. The linear ranges of apixins, aesculin and aescine were 6.0 ~ 96mg.L-1 (r = 0.9997, n = 5), 7 ~ 112mg.L- The average recoveries were 101.74%, 100.92% and 102.44%, respectively, with corresponding RSDs of 2.18%, 1.97% and 2.66% (r = 0.9994, n = n = 9). Conclusion This experiment provides a quick and easy method for the simultaneous quantification of otherapin, aesculin and aesculetin.