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目的探讨二甲基乙酰胺(DMAC)诱导人胚肺成纤维细胞(HELF凋亡)的可能机制。方法用MTT法检测细胞生长抑制率,用Hoechst染色法检测细胞凋亡,用Western blot法检测Bcl-2、Caspase-3蛋白表达。结果DMAC处理HELF细胞24 h均可诱导细胞发生凋亡,并呈现明显的剂量-效应关系;DMAC诱导HELF细胞凋亡过程中伴随着Bcl-2蛋白表达下降、Caspase-3蛋白表达增加。结论 DMAC可诱导HELF细胞凋亡,其机制可能与Bcl-2蛋白表达下降从而激活Caspase-3的表达有关。
Objective To investigate the possible mechanism of dimethylacetamide (DMAC) inducing human embryonic lung fibroblasts (HELF) apoptosis. Methods MTT assay was used to detect the cell growth inhibition rate. Hoechst staining was used to detect cell apoptosis. Western blot was used to detect the protein expression of Bcl-2 and Caspase-3. Results The apoptosis of HELF cells was induced by DMAC for 24 h, and showed a dose-effect relationship. The apoptosis of HELF cells induced by DMAC was accompanied by the decrease of Bcl-2 protein expression and the increase of Caspase-3 protein expression. Conclusion DMAC can induce the apoptosis of HELF cells. The mechanism may be related to the decrease of Bcl-2 protein expression and the activation of Caspase-3 expression.