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目的:探讨双氢青蒿素对佐剂性关节炎(AA)大鼠外周血T细胞的调节作用。方法:采用佐剂性关节炎动物模型,将体重160～180 g的SD大鼠随机设为6组:正常对照组、模型对照组、甲氨蝶呤对照组(1 mg.kg-1)、双氢青蒿素高、中、低剂量(11.2,5.6,2.8 mg.kg-1)组,造模2周后灌胃给药,连续给药28 d,并采用流式细胞术检测外周血T淋巴细胞亚群、酶联免疫法检测血清白介素-4(IL-4)、干扰素-γ(IFN-γ)的水平。结果:相较于正常组,模型组外周血CD4+T,CD4+T/CD8+T和血清IFN-γ水平显著升高(P<0.01),血清IL-4水平显著降低(P<0.01),CD3+T,CD8+T则无明显变化;高、中剂量双氢青蒿素组CD4+T所占百分比分别是(34.81±3.80)%,(34.92±5.14)%,CD4+T/CD8+T分别是2.21±0.43,2.27±0.48,与模型组相比显著降低(P<0.05,P<0.01);双氢青蒿素高、中、低剂量组血清INF-γ水平分别是(15.90±2.05),(16.27±2.11),(18.15±2.15)ng.L-1,与模型组相比显著降低(P<0.01);IL-4水平分别是(40.21±4.89),(40.04±4.56),(34.81±4.02)ng.L-1,与模型组相比显著升高(P<0.01),其调节作用在2.8～5.6 mg.kg-1范围呈剂量依赖性(P<0.05)。结论:双氢青蒿素能有效改善AA大鼠T细胞功能紊乱状况,为其应用于类风湿关节炎治疗奠定实验基础。
Objective: To investigate the regulatory effect of dihydroartemisinin on peripheral blood T cells in adjuvant arthritis (AA) rats. Methods: The animal models of adjuvant arthritis were used. The SD rats weighing 160-180 g were randomly divided into 6 groups: normal control group, model control group, methotrexate control group (1 mg.kg-1) Dihydroartemisinin groups were given intragastric administration of high, medium and low doses of 11.2, 5.6, 2.8 mg.kg-1 for 2 weeks and continuous administration for 28 days. Peripheral blood was detected by flow cytometry T lymphocyte subsets, and the levels of serum interleukin-4 (IL-4) and interferon-γ (IFN-γ) by enzyme-linked immunosorbent assay. Results: Compared with normal group, the level of CD4 + T, CD4 + T / CD8 + T and serum IFN-γ in peripheral blood of model group were significantly increased (P <0.01) (34.81 ± 3.80)%, (34.92 ± 5.14)%, CD4 + T / CD8, CD8 + T and CD8 + T were significantly higher in high and medium doses of dihydroartemisinin group + T were 2.21 ± 0.43 and 2.27 ± 0.48, respectively, which were significantly lower than those in the model group (P <0.05, P <0.01). Serum INF-γ levels in high, middle and low doses of dihydroartemisinin were (15.90 (P <0.01); IL-4 levels were (40.21 ± 4.89) and (40.04 ± 4.56), respectively (P <0.01) ) And (34.81 ± 4.02) ng.L-1, respectively, which were significantly increased compared with the model group (P <0.01). The effect was dose-dependent in the range of 2.8-5.6 mg.kg-1 (P <0.05). Conclusion: Dihydroartemisinin can effectively improve the function of T cell dysfunction in AA rats and lay the experimental foundation for its application in the treatment of rheumatoid arthritis.