论文部分内容阅读
目的:探讨性激素浓度及其受体和雌激素受体β(ERβ)基因多态性与青年人胃腺癌发生发展的关系。方法:以原发性青年胃腺癌患者男女各108例为实验组,随机抽取住院手术胃溃疡患者男女各80例为对照组,用放射免疫法(RIA)检测两组血清性激素水平;用免疫组织化学法(SP法)检测两组雌激素受体(estrogen receptor,ER)表达状况,并用聚合酶链反应-限制性片断长度多态性技术(PCR-RFLP)检测ERβ基因多态性。结果:实验组血清雄激素高于对照组,但雌激素低于对照组,差异均有统计学意义(P<0.0001)。实验组男女性患者,在TNM分期之间差异无统计学意义(P>0.05),但雄激素递减,雌激素递增;而随分化程度降低、淋巴结转移阴性和阳性之间,差异均有统计学意义(P<0.01)。对照组ERα和ERβ均不表达,而实验组ERα和ERβ阳性率分别为57.4%(124/216)和15.7%(34/216),经统计学处理具有显著性差异(P<0.0001),其中分化程度低、有淋巴结转移者、浸润程度深的阳性率高于分化程度高、无淋巴结转移者、浸润程度浅的,且TNMⅢ期高于Ⅰ、Ⅱ期(P<0.001)。PCR-RFLP检测中,用Rsa I酶切显示实验组RR和Rr基因型显著高于rr型,并以RR基因型最高(P<0.0001),且分化程度低、有淋巴结转移和浸润越深者RR高,TNM分期Ⅲ期高于Ⅰ、Ⅱ期(P<0.0001);Alu I酶切显示各基因型差异无统计学意义(P>0.05)。结论:血清性激素水平改变是影响胃腺癌变和易感性的重要因素之一,ERβ基因多态性可能与胃癌的发生发展密切相关。
Objective: To investigate the relationship between sex hormone concentration and its receptor and estrogen receptor β (ERβ) gene polymorphisms and the occurrence and development of gastric adenocarcinoma in young people. Methods: Eighty male and female patients with primary gastric adenocarcinoma of young patients were randomly divided into experimental group and 80 male and female hospitalized patients with gastric ulcer. The levels of serum sex hormone in the two groups were detected by radioimmunoassay (RIA) The expression of estrogen receptor (ER) was detected by chemical method (SP method). The polymorphism of ERβ gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The level of serum androgen in experimental group was higher than that in control group, but the level of estrogen in experimental group was lower than that in control group (P <0.0001). The male and female patients in the experimental group had no significant difference in the TNM stage (P> 0.05), but the androgen decreased and the estrogen increased. With the decrease of the differentiation degree and the negative and positive lymph node metastasis, the difference was statistically significant Significance (P <0.01). The positive rates of ERα and ERβ in the experimental group were 57.4% (124/216) and 15.7% (34/216) respectively, and there was a significant difference (P <0.0001) between the two groups The positive rate of those with low degree of differentiation and lymph node metastasis was higher than those with high degree of differentiation, without lymph node metastasis, and with a slight degree of infiltration. TNM stage Ⅲ was higher than stage Ⅰ and Ⅱ (P <0.001). PCR-RFLP detection, Rsa I digestion showed that the experimental group RR and Rr genotypes were significantly higher than the rr type, and the highest RR genotype (P <0.0001), and the low degree of differentiation, lymph node metastasis and invasion deeper RR was higher, TNM stage Ⅲ was higher than stage Ⅰ, Ⅱ (P <0.0001); Alu I digestion showed no significant difference in genotypes (P> 0.05). Conclusion: The change of serum sex hormone level is one of the important factors that affect the carcinogenesis and susceptibility of gastric adenocarcinoma. The polymorphism of ERβ gene may be closely related to the occurrence and development of gastric cancer.