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目的:建立测定大鼠尿中拉米夫定浓度的毛细管电泳方法,并应用此方法研究拉米夫定经大鼠尿排泄的情况。方法:尿样经沉淀蛋白处理后,采用未涂层熔融石英毛细管柱(45 cm×75μm,有效长度为36.7 cm)分离,运行缓冲液为20 mmol.L-1磷酸盐缓冲液(pH2.5),分离电压为15 kV,检测波长为270 nm,温度为25℃。结果:测定大鼠尿中拉米夫定的线性范围为20.0～1000μg.mL-1(r=0.9994),日内和日间精密度均小于5.8%,准确度为93.8%～101.9%,提取回收率为90.3%～94.8%。大鼠灌胃给予拉米夫定后24 h内尿中原形药物的累积排泄量约占给药剂量的49.8%。结论:该方法简便、快速、经济,可用于拉米夫定在大鼠体内的排泄研究。
OBJECTIVE: To establish a capillary electrophoresis method for the determination of lamivudine in urine of rats and to study the urinary excretion of lamivudine in rats by this method. Methods: Urine samples were separated by sedimentation, uncoated fused silica capillary column (45 cm × 75 μm, effective length 36.7 cm). The running buffer was 20 mmol·L -1 phosphate buffer (pH 2.5 ), The separation voltage is 15 kV, the detection wavelength is 270 nm and the temperature is 25 ℃. Results: The linear range of lamivudine in urine of rats was 20.0-1000 μg.mL-1 (r = 0.9994). The intra-and inter-day precision was less than 5.8% and the accuracy was 93.8% -101.9% The rate was 90.3% ~ 94.8%. The cumulative excretion of urinary prototype drugs within 24 h after oral gavage of lamivudine accounted for about 49.8% of the administered dose. Conclusion: The method is simple, rapid and economical and can be used for excretion of lamivudine in rats.