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目的制备环巴胺固体脂质纳米粒(Cyc-SLNs),并考察其理化性质和对Hedgehog信号通路的抑制作用。方法采用薄膜超声法制备Cyc-SLNs,并考察其对B16F10黑色素瘤细胞增殖、细胞周期、凋亡和Hedgehog信号通路靶基因Gli1表达的影响。结果制得的Cyc-SLNs呈平整的球形,平均粒径、多分散指数(PDI)、Zeta电位和平均包封率分别为126.5±2.1 nm、0.198±0.007、23.5±0.7 m V、91.59%±0.37%;Cyc-SLNs对B16F10细胞增殖分裂的抑制、凋亡的促进和Gli1表达的下调均强于环巴胺。结论成功制备了抗肿瘤细胞效果强于环巴胺的固体脂质纳米粒Cyc-SLNs。
OBJECTIVE To prepare cyclopamine solid lipid nanoparticles (PLL-SLNs) and study their physicochemical properties and inhibitory effect on Hedgehog signaling pathway. Methods Cyclo-SLNs were prepared by membrane ultrasonography and their effects on the proliferation, cell cycle, apoptosis and Gli1 expression of Hedgehog signaling pathway in B16F10 melanoma cells were investigated. Results The Cyc-SLNs obtained showed a flat spherical shape. The average particle diameter, polydispersity index (PDI), zeta potential and average encapsulation efficiency were 126.5 ± 2.1 nm, 0.198 ± 0.007, 23.5 ± 0.7 mV and 91.59% ± 0.37%. Cyc-SLNs inhibited the proliferation and division of B16F10 cells, promoted the apoptosis and decreased the expression of Gli1. Conclusion Cyclo-SLNs, a solid lipid nanoparticle with antitumor effect better than cyclopamine, were successfully prepared.